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when v=vmax/2，Km =（S） 1.why？？Need Explaination. What is V and what is Vmax？how is their relationship and how they affect each other？Detail explanation please. The Fomular：V/E total = K cat. 2. What Is E total？？？need definition of E total.Detail explanation please

when v=vmax/2，Km =（S） 1.why？？Need Explaination. What is V and what is Vmax？how is their relationship and how they affect each other？Detail explanation please. The Fomular：V/E total = K cat. 2. What Is E total？？？need definition of E total.Detail explanation please

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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when v=vmax/2，Km =（S） 1.why？？Need Explaination. What is V and what is Vmax？how is their relationship and how they affect each other？Detail explanation please. The Fomular：V/E total = K cat. 2. What Is E total？？？need definition of E total.Detail explanation please.
Significance of KM and Vmax When V = Vmax/2, KM = [S] Consider a case in which the reaction E +S →ES takes place more frequently than ES → E + P ● ● ● ● • k_₁ is greater than k₂ (k_₁ >> k₂) is related to the turnover number of an enzyme Turnover number: Number of moles of substrate that react to form product per mole of enzyme per second Also called Keat or kp Vm max ved. ● V [E₁] = turnover number = kat
Transcribed Image Text:Significance of KM and Vmax When V = Vmax/2, KM = [S] Consider a case in which the reaction E +S →ES takes place more frequently than ES → E + P ● ● ● ● • k_₁ is greater than k₂ (k_₁ >> k₂) is related to the turnover number of an enzyme Turnover number: Number of moles of substrate that react to form product per mole of enzyme per second Also called Keat or kp Vm max ved. ● V [E₁] = turnover number = kat
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