Km and Vmax obtained from the Lineweaver-Burk plot and linear regression analysis for the data in Table 2 are 16.9 mM and 503 mM respectively. Compare these values to the values obtained Vmax = 525.3 µM/sec    Km = 20.70 µM . Which method, nonlinear regression or linear regression, is more tolerant to an experimental error? Note that lower substrate concentrations, which often have the greater error, have a greater effect on the slope of the line in the Lineweaver-Burk plot.

Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
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Chapter1: Biochemistry: An Evolving Science
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[S] (mM)

v0 (mM/sec)

6

150

17

250

29

260

40

360

50

350

60

400

72

403

82

425

92

430

103

442

115

455

125

450

Km and Vmax obtained from the Lineweaver-Burk plot and linear regression analysis for the data in Table 2 are 16.9 mM and 503 mM respectively. Compare these values to the values obtained Vmax = 525.3 µM/sec    Km = 20.70 µM

. Which method, nonlinear regression or linear regression, is more tolerant to an experimental error?

Note that lower substrate concentrations, which often have the greater error, have a greater effect on the slope of the line in the Lineweaver-Burk plot.

 

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