7. Bromophenol Blue, a reagent or standard that is usually used to determine the void volume of a gel filtration chromatography column, has an approximate molecular weight of 2,000,000 Da. 8. The recommended range of sample volume to be loaded onto the gel filtration chromatography column is within 5-10 percent of the column's total volume. 9. A calculated partition coefficient (Kav) that is less than 1 indicates that no solute or protein entered the pores of the gel filtration chromatography column resin/beads.

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
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Chapter1: The Human Body: An Orientation
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Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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7. Bromophenol Blue, a reagent or standard that is usually used to determine the void volume of a gel
filtration chromatography column, has an approximate molecular weight of 2,000,000 Da.
8. The recommended range of sample volume to be loaded onto the gel filtration chromatography
column is within 5-10 percent of the column's total volume.
9. A calculated partition coefficient (Kav) that is less than 1 indicates that no solute or protein entered
the pores of the gel filtration chromatography column resin/beads.
10. In a medium with pH 4.5, a protein with an IpH of 5.5 is expected to migrate towards the anode.
11. Sodium dodecyl sulfate binds strongly to the thermally denatured protein opening it up into a rod-
shaped structure with a series of negatively charged molecules along the polypeptide chain.
12. Aside from SDS and B-mercaptoethanol, the sample buffer also contains an ionizable tracking dye,
usually Coomassie Brilliant Blue R-250, that allows the electrophoretic run to be monitored.
Transcribed Image Text:7. Bromophenol Blue, a reagent or standard that is usually used to determine the void volume of a gel filtration chromatography column, has an approximate molecular weight of 2,000,000 Da. 8. The recommended range of sample volume to be loaded onto the gel filtration chromatography column is within 5-10 percent of the column's total volume. 9. A calculated partition coefficient (Kav) that is less than 1 indicates that no solute or protein entered the pores of the gel filtration chromatography column resin/beads. 10. In a medium with pH 4.5, a protein with an IpH of 5.5 is expected to migrate towards the anode. 11. Sodium dodecyl sulfate binds strongly to the thermally denatured protein opening it up into a rod- shaped structure with a series of negatively charged molecules along the polypeptide chain. 12. Aside from SDS and B-mercaptoethanol, the sample buffer also contains an ionizable tracking dye, usually Coomassie Brilliant Blue R-250, that allows the electrophoretic run to be monitored.
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