4. RNA hydrolysis As discussed in class, we can predict how much of RNA we expect to be in a reactive form using general chemistry calculations(Henderson Hasselbach). a) Calculate the percent of RNA at pH-8 that will have the 3'OH in its reactive alkoxide form (O-). The pKa of the 3'OH is approximately 13. b) Now redo the calculation to get the percent O-/OH at pH=6, and pH=10. c) What conditions favor more reactive RNA? How could we use a catalyst to enable more RNA to be in its reactive form?
4. RNA hydrolysis As discussed in class, we can predict how much of RNA we expect to be in a reactive form using general chemistry calculations(Henderson Hasselbach). a) Calculate the percent of RNA at pH-8 that will have the 3'OH in its reactive alkoxide form (O-). The pKa of the 3'OH is approximately 13. b) Now redo the calculation to get the percent O-/OH at pH=6, and pH=10. c) What conditions favor more reactive RNA? How could we use a catalyst to enable more RNA to be in its reactive form?
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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