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4. RNA hydrolysis As discussed in class, we can predict how much of RNA we expect to be in a reactive form using general chemistry calculations(Henderson Hasselbach). a) Calculate the percent of RNA at pH-8 that will have the 3'OH in its reactive alkoxide form (O-). The pKa of the 3'OH is approximately 13. b) Now redo the calculation to get the percent O-/OH at pH=6, and pH=10. c) What conditions favor more reactive RNA? How could we use a catalyst to enable more RNA to be in its reactive form?

4. RNA hydrolysis As discussed in class, we can predict how much of RNA we expect to be in a reactive form using general chemistry calculations(Henderson Hasselbach). a) Calculate the percent of RNA at pH-8 that will have the 3'OH in its reactive alkoxide form (O-). The pKa of the 3'OH is approximately 13. b) Now redo the calculation to get the percent O-/OH at pH=6, and pH=10. c) What conditions favor more reactive RNA? How could we use a catalyst to enable more RNA to be in its reactive form?

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Need help with question 4, thank you 

4:47 PM Wed Mar 27 ... lyceum.bates.edu 5 of 8 primer 3'-end WAT1 2.12 H₂O dCTP 2.25 (2.86) 2.11 (N/A) - 2.25 (2.44) MgA 2.35 (2.45) (2.26) 2.01 (2.31) 3.79 MgB 1.87' 1.86 (3.87) 1.98 (2.50) (2.41) (2.39) 1.86 1.97 1.87 (2.24) (2.45) (2.34) WAT2 D654 A476 D475 4. RNA hydrolysis As discussed in class, we can predict how much of RNA we expect to be in a reactive form using general chemistry calculations(Henderson Hasselbach). a) Calculate the percent of RNA at pH=8 that will have the 3'OH in its reactive alkoxide form (O-). The pKa of the 3'OH is approximately 13. b) Now redo the calculation to get the percent O-/OH at pH=6, and pH=10. c) What conditions favor more reactive RNA? How could we use a catalyst to enable more RNA to be in its reactive form? ດ 92%
Transcribed Image Text:4:47 PM Wed Mar 27 ... lyceum.bates.edu 5 of 8 primer 3'-end WAT1 2.12 H₂O dCTP 2.25 (2.86) 2.11 (N/A) - 2.25 (2.44) MgA 2.35 (2.45) (2.26) 2.01 (2.31) 3.79 MgB 1.87' 1.86 (3.87) 1.98 (2.50) (2.41) (2.39) 1.86 1.97 1.87 (2.24) (2.45) (2.34) WAT2 D654 A476 D475 4. RNA hydrolysis As discussed in class, we can predict how much of RNA we expect to be in a reactive form using general chemistry calculations(Henderson Hasselbach). a) Calculate the percent of RNA at pH=8 that will have the 3'OH in its reactive alkoxide form (O-). The pKa of the 3'OH is approximately 13. b) Now redo the calculation to get the percent O-/OH at pH=6, and pH=10. c) What conditions favor more reactive RNA? How could we use a catalyst to enable more RNA to be in its reactive form? ດ 92%
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