i) Write down the equation derived from your Excel generated standard curve , figure legend and describe its components; ii) provide the values of the absorbance data of the unknown sample (do NOT show the absorbance data of the glycine standards). Show all details of the working out of your calculation. Indicate all units! Provide the answer with two decimals precision
i) Write down the equation derived from your Excel generated standard curve , figure legend and describe its components; ii) provide the values of the absorbance data of the unknown sample (do NOT show the absorbance data of the glycine standards). Show all details of the working out of your calculation. Indicate all units! Provide the answer with two decimals precision
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
Related questions
Question
- i) Write down the equation derived from your Excel generated standard curve , figure legend and describe its components;
- ii) provide the values of the absorbance data of the unknown sample (do NOT show the absorbance data of the glycine standards). Show all details of the working out of your calculation. Indicate all units! Provide the answer with two decimals precision
Transcribed Image Text:Excel File Edit View Insert Format Tools Data Window Help
2.0
Formulas Data
Home
Paste
Chart 3
1 Sample
2 Blank
3 Standard 1
4 Standard 2
5 Standard 3
6 Standard 4
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7 Glycine Unknown
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Sheet1
Calibri (Body)
B I U v
✓ fx
0
0.02
0.04
0.06
0.08
1
B
Glucose Conc. (mm)
0
0.02
0.04
0.06
0.08
V 16 V
Glucose Conc. (mm)
+
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Accessibility: Investigate
| 4
V
Α΄ Α΄
Av
A570 (1)
0
0.11
0.196
0.303
0.39
0.393
C
Average A570
0
0.111
0.206
0.301
0.397
9
Review View
三 三 三
A570(2)
0
0.112
0.217
0.288
0.393
0.417
D
Absorbance (570nm)
0.45
0.4
0.35
0.3
0.25
0.2
0.15
0.1
0.05
Automate
A570(3)
0
0.11
0.206
0.312
0.41
0.405
0
General
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%
A
Book1
0.02
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Average A570
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0.11
0.206
0.301
.00
←0
.00 →.0
0.397
0.405
Glucose Standard Curve
Absorbance 570nm= 5.0233 X glucose concentration (mm)
Tell me
Cell
Conditional Format
Formatting as Table Styles
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0.04
Glucose concentration (mm)
0.06
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0.08
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APO
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