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II. Lipids from an organic sample are extracted separately by column chromatography using the ff eluants: 1st eluant chloroform: methanol: water (6:3:1) 2nd eluant methanol: chloroform (9:1) 3rd eluant - petroleum ether: ethyl ether (1:1) Test results for each eluate are tabulated below. Identify the possible lipid present in each eluate. Test Reagents 1. KHSO4 2. HNO3, (NH4)3M0O4 3. CHCl3, acetic anhydride, conc. H₂SO4 4. a-naphthol, conc. H₂SO4 5. Hydroxylamine HCI, FeCl3 6. ninhydrin reagent POSSIBLE LIPIDS: Triacylglycerol, cholesterol, sphingomyelin, lecithin 1st eluate + + + + 2nd eluate + 3rd eluate + +

II. Lipids from an organic sample are extracted separately by column chromatography using the ff eluants: 1st eluant chloroform: methanol: water (6:3:1) 2nd eluant methanol: chloroform (9:1) 3rd eluant - petroleum ether: ethyl ether (1:1) Test results for each eluate are tabulated below. Identify the possible lipid present in each eluate. Test Reagents 1. KHSO4 2. HNO3, (NH4)3M0O4 3. CHCl3, acetic anhydride, conc. H₂SO4 4. a-naphthol, conc. H₂SO4 5. Hydroxylamine HCI, FeCl3 6. ninhydrin reagent POSSIBLE LIPIDS: Triacylglycerol, cholesterol, sphingomyelin, lecithin 1st eluate + + + + 2nd eluate + 3rd eluate + +

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Lipids from an organic sample are extracted separately by column chromatography using the ff eluants: 1st eluant - chloroform: methanol: water (6:3:1) 2nd eluant - methanol: chloroform (9:1) 3rd eluant – petroleum ether: ethyl ether (1:1) Test results for each eluate are tabulated below. Identify the possible lipid present in each eluate. Test Reagents 1. KHSO4 2. HNO3,(NH4)3MoO4 3. CHCl3, acetic anhydride, conc. H2SO4 4. -naphthol, conc. H2SO4 5. Hydroxylamine HCl, FeCl3 6. ninhydrin reagent POSSIBLE LIPIDS: Triacylglycerol, cholesterol, sphingomyelin, lecithin
II. Lipids from an organic sample are extracted separately by column chromatography using the ff eluants: 1st eluant chloroform: methanol: water (6:3:1) 2nd eluant methanol: chloroform (9:1) 3rd eluant - petroleum ether: ethyl ether (1:1) Test results for each eluate are tabulated below. Identify the possible lipid present in each eluate. Test Reagents 1. KHSO4 2. HNO3, (NH4)3M0O4 3. CHCl3, acetic anhydride, conc. H₂SO4 4. a-naphthol, conc. H₂SO4 5. Hydroxylamine HCI, FeCl3 6. ninhydrin reagent POSSIBLE LIPIDS: Triacylglycerol, cholesterol, sphingomyelin, lecithin 1st eluate + + + + 2nd eluate + 3rd eluate + +
Transcribed Image Text:II. Lipids from an organic sample are extracted separately by column chromatography using the ff eluants: 1st eluant chloroform: methanol: water (6:3:1) 2nd eluant methanol: chloroform (9:1) 3rd eluant - petroleum ether: ethyl ether (1:1) Test results for each eluate are tabulated below. Identify the possible lipid present in each eluate. Test Reagents 1. KHSO4 2. HNO3, (NH4)3M0O4 3. CHCl3, acetic anhydride, conc. H₂SO4 4. a-naphthol, conc. H₂SO4 5. Hydroxylamine HCI, FeCl3 6. ninhydrin reagent POSSIBLE LIPIDS: Triacylglycerol, cholesterol, sphingomyelin, lecithin 1st eluate + + + + 2nd eluate + 3rd eluate + +
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