A student used 3.9g of liver sample and extracted all the lipids into a total of 1ml of CHCl3. 5 microliters of the total sample was transfered in to a fresh tube and 2ml of ethanol and 2ml of acid-ferric chloride was added. After careful mixing and 30minutes of incubation, absorption of the sample was measured at 550nm. Absorption was 0.55. What is the cholesterol content in liver in micromoles of cholesterol per gram of liver?
A student used 3.9g of liver sample and extracted all the lipids into a total of 1ml of CHCl3. 5 microliters of the total sample was transfered in to a fresh tube and 2ml of ethanol and 2ml of acid-ferric chloride was added. After careful mixing and 30minutes of incubation, absorption of the sample was measured at 550nm. Absorption was 0.55. What is the cholesterol content in liver in micromoles of cholesterol per gram of liver?
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Question
A student used 3.9g of liver sample and extracted all the lipids into a total of 1ml of CHCl3. 5 microliters of the total sample was transfered in to a fresh tube and 2ml of ethanol and 2ml of acid-ferric chloride was added. After careful mixing and 30minutes of incubation, absorption of the sample was measured at 550nm. Absorption was 0.55. What is the cholesterol content in liver in micromoles of cholesterol per gram of liver?
A) 6.243
B) 24.972
C) 486.98
D) 122.05
E) 4.86
![Absorbance
1.0
0.8
Cholesterol Standard Curve
y = -0.017 +0.0010945x
0.6
ㄖ
0.4
0.2
0.0
0
200
400
6.243
24.972
486.98
☐ 122.05
4.86
600 800
[cholesterol] (nmol/tube)](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2Fca7515a8-b44b-40fa-83f2-449e6ef6ad98%2F55fea28a-a2a8-446a-aad7-a8dd68130db0%2F98u0z8k_processed.jpeg&w=3840&q=75)
Transcribed Image Text:Absorbance
1.0
0.8
Cholesterol Standard Curve
y = -0.017 +0.0010945x
0.6
ㄖ
0.4
0.2
0.0
0
200
400
6.243
24.972
486.98
☐ 122.05
4.86
600 800
[cholesterol] (nmol/tube)
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