You are told to prepare 200 mL of fixative solution containing 30% formaldehyde (vol/vol), 1X glycerol and 2.5 mM sodium chloride (NaCl). You find a stock of 100% pure formaldehyde (liquid), a 10X glycerol stock (liquid), plenty of water and a stock solution of 5M NaCl. There are also all kinds of pipettes, cylinders and beakers to measure volumes. How can the fixative solution be made (describe the process, include calculation concepts)?
You are told to prepare 200 mL of fixative solution containing 30% formaldehyde (vol/vol), 1X glycerol and 2.5 mM sodium chloride (NaCl). You find a stock of 100% pure formaldehyde (liquid), a 10X glycerol stock (liquid), plenty of water and a stock solution of 5M NaCl. There are also all kinds of pipettes, cylinders and beakers to measure volumes. How can the fixative solution be made (describe the process, include calculation concepts)?
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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You are told to prepare 200 mL of fixative solution containing 30% formaldehyde (vol/vol), 1X glycerol and 2.5 mM sodium chloride (NaCl). You find a stock of 100% pure formaldehyde (liquid), a 10X glycerol stock (liquid), plenty of water and a stock solution of 5M NaCl. There are also all kinds of pipettes, cylinders and beakers to measure volumes.
How can the fixative solution be made (describe the process, include calculation concepts)?
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