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Report how to make a 250.mL of 50pM sodium acetate and 0.01p EDTA using solid sodium acetate and 10µM EDTA.

Report how to make a 250.mL of 50pM sodium acetate and 0.01p EDTA using solid sodium acetate and 10µM EDTA.

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Title: Preparing a 250 mL Solution of 50 µM Sodium Acetate and 0.01 µM EDTA Instructions: To prepare a 250 mL solution of 50 µM sodium acetate and 0.01 µM EDTA, follow these steps using solid sodium acetate and a 10 µM EDTA solution. Materials Needed: - Solid sodium acetate - 10 µM EDTA solution - Distilled water - Balance - Volumetric flask (250 mL) - Pipette - Stirring rod Procedure: 1. **Calculate the required mass of sodium acetate:** - Use the molecular weight of sodium acetate to determine the mass needed for 50 µM concentration in 250 mL. - Dissolve the calculated mass in a portion of distilled water. 2. **Add EDTA:** - Use a pipette to add the required volume of 10 µM EDTA to achieve a final concentration of 0.01 µM in the 250 mL solution. 3. **Mixing:** - Transfer the solutions to a 250 mL volumetric flask. - Add distilled water to reach the final volume of 250 mL. - Mix thoroughly using a stirring rod to ensure homogeneity. 4. **Storage:** - Store the solution as required for your experiment or application. This preparation guide ensures precision in creating the specified solution concentration for experimental needs. Always follow safety protocols while handling chemicals.
Transcribed Image Text:Title: Preparing a 250 mL Solution of 50 µM Sodium Acetate and 0.01 µM EDTA Instructions: To prepare a 250 mL solution of 50 µM sodium acetate and 0.01 µM EDTA, follow these steps using solid sodium acetate and a 10 µM EDTA solution. Materials Needed: - Solid sodium acetate - 10 µM EDTA solution - Distilled water - Balance - Volumetric flask (250 mL) - Pipette - Stirring rod Procedure: 1. **Calculate the required mass of sodium acetate:** - Use the molecular weight of sodium acetate to determine the mass needed for 50 µM concentration in 250 mL. - Dissolve the calculated mass in a portion of distilled water. 2. **Add EDTA:** - Use a pipette to add the required volume of 10 µM EDTA to achieve a final concentration of 0.01 µM in the 250 mL solution. 3. **Mixing:** - Transfer the solutions to a 250 mL volumetric flask. - Add distilled water to reach the final volume of 250 mL. - Mix thoroughly using a stirring rod to ensure homogeneity. 4. **Storage:** - Store the solution as required for your experiment or application. This preparation guide ensures precision in creating the specified solution concentration for experimental needs. Always follow safety protocols while handling chemicals.
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