Calculate the amount of BSA you will add to the tube to get the concentrations listed on the chart. Your stock tube is 2mg/mL. The amount of water will be the amount to bring your total volume up to 100uL. Standard Concentration BSA H2O Total volume A 200 ug/mL 100uL B 150 ug/mL 100uL C 100 ug/mL 100uL D 50 ug/mL 100uL E 20 ug/mL 100uL F 10 ug/mL 100uL G 5 ug/mL 100uL H 0 ug/mL 0 uL 100uL 100uL

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Scientific Notation

We have to deal with quantities that are extremely large or very small. For example- I may have 6,500,000,000 red blood cells, or the diameter of an iron atom is 0.000000011 inches. Numbers with many zeros can be inexpedient to work with, so scientists use scientific notation. It represents very large numbers or very small numbers in a simple form. We know that whole numbers can be extended to infinity, but we cannot write such large numbers on paper. Numbers that present in millions of places after the decimal point need to be represented simply. Thus, it is difficult to represent a few numbers in their extended form. Therefore, we use scientific notation.