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How would I calculate the amount of each component in each solution?  Stock solutions provided: 0.5 M EDTA, pH 8 10% bromophenol blue 10% xylene cyanol Sucrose powder Tris powder Glacial acetic acid  Solution A: 50 mL of 50x Tris Acetate EDTA (TAE) Buffer 2M Tris Base 50mM EDTA, pH 8 11.4% (v/v) glacial acetic acid Solution B: 1L of 1x TAE Buffer 40mM Tris-acetate 1mM EDTA Solution C: 5mL of 10x Loading Dye 0.25% (w/v) bromophenol blue 0.25% (w/v) xylene cyanol 66.5% (w/v) sucrose 100mM EDTA, pH 8

How would I calculate the amount of each component in each solution?  Stock solutions provided: 0.5 M EDTA, pH 8 10% bromophenol blue 10% xylene cyanol Sucrose powder Tris powder Glacial acetic acid  Solution A: 50 mL of 50x Tris Acetate EDTA (TAE) Buffer 2M Tris Base 50mM EDTA, pH 8 11.4% (v/v) glacial acetic acid Solution B: 1L of 1x TAE Buffer 40mM Tris-acetate 1mM EDTA Solution C: 5mL of 10x Loading Dye 0.25% (w/v) bromophenol blue 0.25% (w/v) xylene cyanol 66.5% (w/v) sucrose 100mM EDTA, pH 8

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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How would I calculate the amount of each component in each solution? 

Stock solutions provided:

  • 0.5 M EDTA, pH 8
  • 10% bromophenol blue
  • 10% xylene cyanol
  • Sucrose powder
  • Tris powder
  • Glacial acetic acid 

Solution A: 50 mL of 50x Tris Acetate EDTA (TAE) Buffer

  • 2M Tris Base
  • 50mM EDTA, pH 8
  • 11.4% (v/v) glacial acetic acid

Solution B: 1L of 1x TAE Buffer

  • 40mM Tris-acetate
  • 1mM EDTA

Solution C: 5mL of 10x Loading Dye

  • 0.25% (w/v) bromophenol blue
  • 0.25% (w/v) xylene cyanol
  • 66.5% (w/v) sucrose
  • 100mM EDTA, pH 8
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