You obtained the following raw data when setting up a Bradford standard curve:AbsorbancyBSA (mg/ml)595nm0.22510.31020.4200.51040.6100.72060.8100.91580.9500.980100.990After blanking against a bradford-dH20 sample, the protein concentration of an unknown sample was determined using the same method and anabsorbancy of 0.594 was obtained.Set up a standard curve, excluding outliers (experimental and statistical) and determine the protein concentration in the unknown sample in mg / ml (up to 3significant figures).

The Bradford curve is used for estimating and analyzing the protein which is present in the given…

Answered: You obtained the following raw data…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Similar questions

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You are given a 27 % (w/v) solution of psicose. Molar mass: psicose (C6H12O6) = 180.156 g/mol. ou have a 0.75 mg/mL solution of bovine serum albumin (BSA). You add 1 mL of the 0.75 mg/mL BSA solution into a standard cuvette (1 cm path length), set your spectrophotometer to 280 nm and read the absorbance of this BSA solution. Molecular mass of bovine serum albumin = 66.4 kDa. Molar extinction coefficient (e) of BSA = 43824 M-1 cm-1 (at 280 nm). What absorbance reading will you obtain?
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Graph the data of the absorbance and starch concentration on Microsoft excel
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