You assay 0.5 ml of 1/2000 diluted egg white for protein, as described in the practical schedule, and the spectrophotometer reading at 595 nm is 0.6. Using the attached calibration curve(click to enlarge), calculate the concentration of protein in undiluted egg white. Give your answer in mg/ml.
You assay 0.5 ml of 1/2000 diluted egg white for protein, as described in the practical schedule, and the spectrophotometer reading at 595 nm is 0.6. Using the attached calibration curve(click to enlarge), calculate the concentration of protein in undiluted egg white. Give your answer in mg/ml.
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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You assay 0.5 ml of 1/2000 diluted egg white for protein, as described in the practical schedule, and the spectrophotometer reading at 595 nm is 0.6. Using the attached calibration curve(click to enlarge), calculate the concentration of protein in undiluted egg white. Give your answer in mg/ml.
![You assay 0.5 ml of 1/2000 diluted egg
Absorbance @ 595 nm
0.7
0.6
0.5
0.4
0.3
0.2
0.1
0
0
2
4
Bradford calibration curve
6
8
10
12
14
µg protein in assay (Bovine Serum Albumin)
Bradford calibration curve
16
18
20
SUBMIT](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2Fb02a1a1b-0e78-4aa5-b4cd-cc657e4d76cb%2Fab1d4282-545a-45d1-87f7-ab51963b05a4%2Fvso84b_processed.jpeg&w=3840&q=75)
Transcribed Image Text:You assay 0.5 ml of 1/2000 diluted egg
Absorbance @ 595 nm
0.7
0.6
0.5
0.4
0.3
0.2
0.1
0
0
2
4
Bradford calibration curve
6
8
10
12
14
µg protein in assay (Bovine Serum Albumin)
Bradford calibration curve
16
18
20
SUBMIT
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