A 25.0 mL aliquot was taken from a 500.0 mL sample of tonic water containing an unknown amount of quinine and diluted to avolume of 150.0 mL. At 347.5 nm, the fluorescence intensity of the diluted sample was measured as 89.3 on an arbitrary scale.Under similar conditions, a 25.0 ppm standard quinine solution had a fluorescence intensity of 173. Calculate the mass ofquinine in the original tonic water sample.mass:mg* TOOLSx10

Quinine is generally helped to treat malaria that leads by Plasmodium falciparum. This parasite that…

Answered: A 25.0 mL aliquot was taken from a…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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You are to prepare capsules that contain 0.125 g of a drug. You have four partial containers of medicatiog, which weigh 3.2 g, 1.784 g, 2.46 g, and 5.87 g. Assume you have weighed each of the four containers with the same scale, and the accuracy is known to the hundredth gram. a Which amount will need to be rounded? b. Which amount is not as accurate as it should be? c. What is the amount of the medication that will be left over after making as many 0.125 g capsules as possible?
A student performed the fractional distillation of a cyclohexane/toluene mixture. The refractive index of the distillate was measured at 1.4301 with an ambient temperature of 22.0°C. What is the mole percentage of cyclohexane (to 3 sf)? 94.5 Incorrect
20 4. A sample containing ethanol and water was found to have n6 = 1.3400. Calculate the exact percentage of ethanol and water in this example. Show your work. (Hint: Solve this mathematically. Assume a linear relationship between concentration and refractive index. You can make the y-intercept the refractive index of water, and use the slope formula to find the slope. The x axis (concentration) will be from 0% ethanol to 100% ethanol. From this information, you can get the equation of the line, then plug in your numbers from there.)
number 57.) what's the answer
For the serial dilution, your stock solution must have a concentration of 3.5 mg/mL. How much diluent must be added to the 5.3 mg/mL red cell to prepare the stock solution? Show pertinent solution/s. 7. If the red cell suspension is the stock solution, what is being quantified in this test? What diluent/reactant should be used to detect your answer in number 7?
If a solution of DNA is 3 mg/ml, 0.5 mg of DNA would be contained in how many ml?
Assume that you have an “X” solution that you do not know its concentration. But you have other X solutions with known concentrations and you know X solution absorbs maximum light at 575 nm. To calculate the unknown solution concentration, you have done some spectrophotometric measurements and obtained data given below. Prepare a standard curve by using the absorbance of known concentrations (Hint: you can learn how to prepare a standard curve by using Excell from youtube). Calculate the concentration of unknown.
A calibration curve for the absorbance of an unknown is shown below. What would be the concentration of a sample with absorbance of 0.45. Give the answer with the correct unit.
How many moles of KBr are there in 89.5 mL of 0.150 M KBr?
Make a standard curve by using the values given in the table and determine the concentration of unknown sample? Tube 0,5 µg/ µl BSA 0,15 M NaCl Comassi Brillant Blue Solution Absorbance 595 nm 1 0 100 µl 900 µl 0 2 10 µl 90 µl 900 µl 0,105 3 20 µl 80 µl 900 µl 0,233 4 30 µl 70 µl 900 µl 0,41 5 40 µl 60 µl 900 µl 0,455 6 50 µl 50 µl 900 µl 0,589 Unknown Sample 10 µl 90 µl 900 µl 1,2
(b) Table Q1 below provides optical measurements of samples with known concentrations of analyte (ten samples were measured and the average and standard deviation are presented). They are also represented graphically in Figure Q1, below the table. Analyte concentration (nM) 0.1 0.2 0.5 1 2 5 10 15 fluorescence intensity 300000 250000 200000 150000 100000 50000 0 0 Table Q1 Fluorescence intensity (average) 9300 9200 10800 19600 37200 90000 178000 224000 5 [Analyte]/nM 10 Standard deviation 2000 1200 1000 2000 10000 30000 25000 40000 15 Figure Q1. Signal intensity for different analyte concentrations. Error bars are standard deviation. (ii) Calculate the limit of detection (LOD) for this sensor. (iii) A patient sample measured using this biosensor in the diagnostic laboratory generates a signal of 142800. The coefficient of variation of the device is 5%. Explain whether the diagnostic lab would have the confidence to report that the sample has a concentration in analyte of 8 nM or…
Consider the fluorescence spectra for bovine serum albumin (BSA) below. One solution contains phosphate buffered saline (PBS), while the other contains 1M HCL. Why does the fluorescence change the way it does? I.e., why do we observe a higher peak and a shift to the left under the presence of acid?

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