A 25.0 mL aliquot was taken from a 500.0 mL sample of tonic water containing an unknown amount of quinine and diluted to a volume of 150.0 mL. At 347.5 nm, the fluorescence intensity of the diluted sample was measured as 89.3 on an arbitrary scale. Under similar conditions, a 25.0 ppm standard quinine solution had a fluorescence intensity of 173. Calculate the mass of quinine in the original tonic water sample.

Biochemistry
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Chapter1: Biochemistry: An Evolving Science
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A 25.0 mL aliquot was taken from a 500.0 mL sample of tonic water containing an unknown amount of quinine and diluted to a
volume of 150.0 mL. At 347.5 nm, the fluorescence intensity of the diluted sample was measured as 89.3 on an arbitrary scale.
Under similar conditions, a 25.0 ppm standard quinine solution had a fluorescence intensity of 173. Calculate the mass of
quinine in the original tonic water sample.
mass:
mg
* TOOLS
x10
Transcribed Image Text:A 25.0 mL aliquot was taken from a 500.0 mL sample of tonic water containing an unknown amount of quinine and diluted to a volume of 150.0 mL. At 347.5 nm, the fluorescence intensity of the diluted sample was measured as 89.3 on an arbitrary scale. Under similar conditions, a 25.0 ppm standard quinine solution had a fluorescence intensity of 173. Calculate the mass of quinine in the original tonic water sample. mass: mg * TOOLS x10
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