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If you run the Lugol's test on an unknown solution and you get a black color at the end, what does this result tell you?
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- A 25.0 mL aliquot was taken from a 500.0 mL sample of tonic water containing an unknown amount of quinine and diluted to a volume of 150.0 mL. At 347.5 nm, the fluorescence intensity of the diluted sample was measured as 89.3 on an arbitrary scale. Under similar conditions, a 25.0 ppm standard quinine solution had a fluorescence intensity of 173. Calculate the mass of quinine in the original tonic water sample. mass: mg * TOOLS x10(MB = methylene blue) What is the concentration of the sample of the substance with the reporter absorbance of 0.40 at 595 nm?You perform a Bradford assay to determine the concentration of isolated α-lactalbumin. You use 50 μL of a two-fold diluted solution of α-lactalbumin in the assay. You generate a standard curve with the following equation for the line: y = 0.163x + 0.082. The absorbance of your sample was 0.674 AU. What is the concentration of α-lactalbumin, in mg/mL, in your sample? Give your answer to three significant figures.
- You have been given a stock solution of dye that has a 10X concentration. You wish to make 750ul of a 1X dilution of this dye. Give the volume of 10X dye that you will use (in ul)Prepare a standard tube that contains 0.2 mL of 2.5 mM lysine. To this tube, add 0.1 mL of the ninhydrin reagent. What is the concentration of lysine in the final reaction mixture?(b) Table Q1 below provides optical measurements of samples with known concentrations of analyte (ten samples were measured and the average and standard deviation are presented). They are also represented graphically in Figure Q1, below the table. Analyte concentration (nM) 0.1 0.2 0.5 1 2 5 10 15 fluorescence intensity 300000 250000 200000 150000 100000 50000 0 0 Table Q1 Fluorescence intensity (average) 9300 9200 10800 19600 37200 90000 178000 224000 5 [Analyte]/nM 10 Standard deviation 2000 1200 1000 2000 10000 30000 25000 40000 15 Figure Q1. Signal intensity for different analyte concentrations. Error bars are standard deviation. (ii) Calculate the limit of detection (LOD) for this sensor. (iii) A patient sample measured using this biosensor in the diagnostic laboratory generates a signal of 142800. The coefficient of variation of the device is 5%. Explain whether the diagnostic lab would have the confidence to report that the sample has a concentration in analyte of 8 nM or…
- why are 2D NMR spectra symmetricalNMR is considered a low-sensitivity spectroscopic technique. What is the physical basis of NMR's insensitivity?What are the x and y values that you have input in the excel? I thought the x would be from the concentation and the y is from the A595 of sample minus A595 of blank but the linear equation that shows up in mine is different. It's y = 0.0443x + 0.0148.
- In a chromatographic analysis, it was found out that distance travelled by the alcohol solvent is 5 cm away from the finish line. The distance travelled from the starting line to finish line is 30 cm. The first amino acid, AA1 travelled 4 cm behind the distance travelled by the alcohol. The second amino acid, AA2 travelled 12 cm short from the solvent front while the third amino acid, AA3 travelled 4 cm ahead of AA2. a) Reproduce the chromatogram. Label. b) Calculate: Rf of AA1 ____ Rf of AA2____ Rf of AA3____You need to calculate the volume of the forward and reverse primers you need to add to each reaction tube using the following information: The stock concentration of each primer is 10 mM. You need a final concentration of 0.3 µM in a 25 µl reaction.Imagine you are conducting an Ames test experiment. Based off your results (shown in pictures), answer these questions: 1. Generate a fully labeled graph/figure depicting the data generated in lab (minimally you should include the data from control, 2-NF, and two different test compounds.) 2. Make a bulleted list of 3 distinct and specific observations/patterns seen in your graph/figure - for each observation, indicate specifically where you should look to find the observation on your figure.