calculate the [PNP] in μM for each of these samples. **Table 1***(WATCH YOUR UNITS OF MEASUREMENT)*| Tube # | PNP (µl) 0.5 mM stock | Buffer (µl) | [PNP] in µM | Trial 1 A405 | Trial 2 A405 ||----------|-----------------------|-------------|-------------|------------------------|------------------------|| Blank | 0 | 1500 | | | || 1 | 10 | 1490 | | | || 2 | 20 | 1480 | | | || 3 | 30 | 1470 | | | || 4 | 40 | 1460 | | | || 5 | 50 | 1450 | | | || 6 | 60 | 1440 | | | || 7 | 75 | 1425 | | | || 8 | 100 | 1400 | | | |*Note that in the PNP column, µM = µmoles/liter.***Explanation of the Table:**This table is structured to present an experiment setup where various concentrations of PNP (p-nitrophenol) solutions are assessed. The PNP solutions are prepared by varying the volume from a 0.5 mM stock solution, while the remaining volume is filled with a buffer to reach a total of 1500 µl per tube. For each tube, the PNP concentration in micromolar (µM) should ideally be calculated and entered in the corresponding column. The absorbance at 405 nm (A405) for two trials is to be recorded. This absorbance reading will help determine the concentration effects on PNP's behavior in the experiment.

Molarity is way of representing the concentration of a solution. Molarity is number of moles of…

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Biochemistry

calculate the [PNP] in μM for each of these samples.

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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