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Biochemistry
starting and ending purity, t

starting and ending purity, t

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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**1.8 Calculations for the Purification of a Recombinant Protein** The purification of a recombinant protein is initiated with 100 liters of a clarified cell lysate (i.e., the cells have been lysed, and the cell debris has been removed to yield a clarified solution), which contains a total protein concentration of 0.36 mg/ml and a recombinant protein concentration of 2.2 U/ml, where U denotes units of biological activity of the recombinant protein. It is known that the completely pure recombinant protein has a specific activity of 40.0 U/mg. The purification process continues until a chromatography step results in 2.0 liters of a fraction containing the protein, with a total protein concentration of 1.11 mg/ml and a recombinant protein concentration of 43.2 U/ml. For the recombinant protein, calculate the starting and ending purity, the starting and ending specific activity, and the percentage yield and fold purification through the chromatography step.
Transcribed Image Text:**1.8 Calculations for the Purification of a Recombinant Protein** The purification of a recombinant protein is initiated with 100 liters of a clarified cell lysate (i.e., the cells have been lysed, and the cell debris has been removed to yield a clarified solution), which contains a total protein concentration of 0.36 mg/ml and a recombinant protein concentration of 2.2 U/ml, where U denotes units of biological activity of the recombinant protein. It is known that the completely pure recombinant protein has a specific activity of 40.0 U/mg. The purification process continues until a chromatography step results in 2.0 liters of a fraction containing the protein, with a total protein concentration of 1.11 mg/ml and a recombinant protein concentration of 43.2 U/ml. For the recombinant protein, calculate the starting and ending purity, the starting and ending specific activity, and the percentage yield and fold purification through the chromatography step.
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