and record the total mass. When you subtract the mass of the empty, dry flask, you will obtain the mass of the solution. Repeat the entire procedure, including rinsing the buret, for a total of three trials. You want to develop your technique so that you can use a buret accurately and reproducibly (precisely). B. Mohr Pipet Using the 25 mL Mohr pipet, carry out the weighing procedure of Part A. Be sure that the pipet is clean and drains correctly, then pre-rinse it with some of your solution. Attempt to deliver exactly 23.00 mL of solution into the dry, weighed Er- lenmeyer flask. If you undershoot or overshoot, record whatever actual volume you finally deliver. Do a total of three trials. Attempt to perfect your technique. C. Volumetric Pipet Carry out the weighing procedure of Part A using a clean, pre-rinsed 25 mL volu- metric pipet to deliver 25.00 mL of solution. If the pipet is marked TD, it is designed to give 25.00 mL by draining with gravity: Do not include the solution that remains in the tip after draining. The tips of the pipets are very fragile. Be careful not to exert pressure on them. Do a total of three trials. D. Volumetric Flask Fill your pre-weighed, dried, volumetric flask with the solution, stopping at the cali- bration mark. This will give you a volume of 25.00 ± 0.02 mL. Weigh the solution- filled container and record the mass. This is the mass of the flask plus the solution "contained," and, by subtracting the mass of the empty flask, you can determine the mass of the solution contained. Empty the contents of the container into the clean, dry, weighed Erlenmeyer flask. Record the mass of the Erlenmeyer flask plus the solution. From this you can calculate the mass of solution "delivered." Repeat this procedure three times. Before you leave the lab, share your results with the others in your group.
and record the total mass. When you subtract the mass of the empty, dry flask, you will obtain the mass of the solution. Repeat the entire procedure, including rinsing the buret, for a total of three trials. You want to develop your technique so that you can use a buret accurately and reproducibly (precisely). B. Mohr Pipet Using the 25 mL Mohr pipet, carry out the weighing procedure of Part A. Be sure that the pipet is clean and drains correctly, then pre-rinse it with some of your solution. Attempt to deliver exactly 23.00 mL of solution into the dry, weighed Er- lenmeyer flask. If you undershoot or overshoot, record whatever actual volume you finally deliver. Do a total of three trials. Attempt to perfect your technique. C. Volumetric Pipet Carry out the weighing procedure of Part A using a clean, pre-rinsed 25 mL volu- metric pipet to deliver 25.00 mL of solution. If the pipet is marked TD, it is designed to give 25.00 mL by draining with gravity: Do not include the solution that remains in the tip after draining. The tips of the pipets are very fragile. Be careful not to exert pressure on them. Do a total of three trials. D. Volumetric Flask Fill your pre-weighed, dried, volumetric flask with the solution, stopping at the cali- bration mark. This will give you a volume of 25.00 ± 0.02 mL. Weigh the solution- filled container and record the mass. This is the mass of the flask plus the solution "contained," and, by subtracting the mass of the empty flask, you can determine the mass of the solution contained. Empty the contents of the container into the clean, dry, weighed Erlenmeyer flask. Record the mass of the Erlenmeyer flask plus the solution. From this you can calculate the mass of solution "delivered." Repeat this procedure three times. Before you leave the lab, share your results with the others in your group.
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Transcribed Image Text:and record the total mass. When you subtract the mass of the empty, dry flask, you
will obtain the mass of the solution.
Repeat the entire procedure, including rinsing the buret, for a total of three trials.
You want to develop your technique so that you can use a buret accurately and
reproducibly (precisely).
B. Mohr Pipet
Using the 25 mL Mohr pipet, carry out the weighing procedure of Part A. Be sure
that the pipet is clean and drains correctly, then pre-rinse it with some of your
solution. Attempt to deliver exactly 23.00 mL of solution into the dry, weighed Er-
lenmeyer flask. If you undershoot or overshoot, record whatever actual volume you
finally deliver. Do a total of three trials. Attempt to perfect your technique.
C. Volumetric Pipet
Carry out the weighing procedure of Part A using a clean, pre-rinsed 25 mL volu-
metric pipet to deliver 25.00 mL of solution. If the pipet is marked TD, it is designed
to give 25.00 mL by draining with gravity: Do not include the solution that remains
in the tip after draining. The tips of the pipets are very fragile. Be careful not to exert
pressure on them. Do a total of three trials.
D. Volumetric Flask
Fill your pre-weighed, dried, volumetric flask with the solution, stopping at the cali-
bration mark. This will give you a volume of 25.00 ± 0.02 mL. Weigh the solution-
filled container and record the mass. This is the mass of the flask plus the solution
"contained," and, by subtracting the mass of the empty flask, you can determine
the mass of the solution contained. Empty the contents of the container into the
clean, dry, weighed Erlenmeyer flask. Record the mass of the Erlenmeyer flask
plus the solution. From this you can calculate the mass of solution "delivered."
Repeat this procedure three times.
Before
you leave the lab, share your results with the others in your group.
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