One of the useful rules of thumb that you may have learned in Biochemistry, is that a 50 g/ml solution of DNA has an absorbance of about 1.0 in a 1 cm cell. A. I have a solution of DNA that has a transmittance of 0.15 when it is placed in a cell with a 200 um pathlength. What is the concentration of DNA in this solution? (in units of g/ml) B. Assume that this DNA is a synthetic oligo containing 25 base pairs, and that the MW of a single base pair has a molecular weight of 330. What is the molar concentration of duplex DNA molecules in the solution? C. What is the Molar absorptivity of DNA in units of L cm (mol base pair) -1 -1
One of the useful rules of thumb that you may have learned in Biochemistry, is that a 50 g/ml solution of DNA has an absorbance of about 1.0 in a 1 cm cell.
A. I have a solution of DNA that has a transmittance of 0.15 when it is placed in a cell with a 200 um pathlength. What is the concentration of DNA in this solution? (in units of g/ml)
B. Assume that this DNA is a synthetic oligo containing 25 base pairs, and that the MW of a single base pair has a molecular weight of 330. What is the molar concentration of duplex DNA molecules in the solution?
C. What is the Molar absorptivity of DNA in units of L cm (mol base pair) -1 -1
In the above question, there should be a correction about the following information:
“One of the useful rules of thumb that you may have learned in Biochemistry, is that a 50 g/ml solution of DNA has an absorbance of about 1.0 in a 1 cm cell.”
As we know the molar extinction coefficients for DNA are: 50 (μg/ml)-1cm-1 for double-stranded DNA at 260 nm
So, I am answering the question considering one of the useful rules of thumb rule in Biochemistry is that a 50 µg/ml solution of double-stranded DNA has an absorbance of about 1.0 in a 1 cm cell at 260 nm wavelengths.
Trending now
This is a popular solution!
Step by step
Solved in 2 steps
