Apohemoglobin (apoHb) is a dimeric globular protein with two vacant heme‐binding. The preparationof apoHb is based on partial hemoglobin (Hb) unfolding to facilitate heme extraction into an organic solvent. What is an appropriate method for removing the heme? a. An acidic buffer to protonate the His axial ligand in the presence of ureaas the denaturing agent. b. An acidic buffer to protonate the His axial ligand in the presence of mercatoethanol as the denaturing agent. c. A basic buffer to deprotonate the His axial ligand in the presence of ureaas the denaturing agent. d. An basic buffer to protonate the His axial ligand in the presence of mercatoethanol as the denaturing agent.

Q: Explain why the proteins involved in blood clotting, fi brin and thrombin, are fi rst synthesized as…

A: Enzymes are the biological catalyst that accelerates the biochemical reaction in the body. They are…

Q: Microcytic anemia could be caused due to both iron deficiency anemia and thalassemia.  could you…

A: Microcytic anaemia is a type of anaemia characterized by smaller-than-normal red blood cells. This…

Q: Rapidly growing Burkholderia bacteria normally produce ammonia as a waste product. The accumulation…

A: Burkholderia complex species are obligatory gram-negative aerobic rods that do not ferment glucose…

Q: A point mutation in Hb diminishes hydrophobicity at the heme. Which of the following impacts would…

A: Hb hemoglobin carries O2 in the blood during the gaseous exchange.

Q: What are the differences in terms of structure and ligand-binding interactions? What are the…

A: GPCRs are a type of transmembrane receptor that consists of seven transmembrane helices that…

Q: What is a secondary transporter? Why are they called secondary?  What is [3 H]Leu? Explain both 3H…

A: It is a type of active transport. It falls across a biological membrane. Here a transporter protein…

Q: Which patch clamp technique should be used if you want to investigate the effect a compound has…

A: The patch clamp technique - a laboratory technique. patch clamp technique used in electrophysiology…

Q: Explain why the negative charges embedded within the filtration membranes are able to repel proteins…

A: In the filtration, the membrane is non specific in a nature and passing of molecules depends on size…

Q: Influenza viruses are surrounded by a membrane that contains a fusion protein, which is activated by…

A: HIV, influenza all have a different mechanism to enter a cell.

Q: The cooperative binding behavior of hemoglobin for oxygen is best explained by...

A: Hemoglobin (Hb) is a protein molecule which is present in red blood cells and carries oxygen from…

Q: 1. What is the overall charge of alkaline phosphatase? a. Which type is used to purify APase?…

A: Alkaline phosphatase Alkaline phosphatase is an enzyme that plays a role in dephosphorylation of…

Q: Compared to adult Hb (HbA), fetal Hb (HbF) has one less histidine in the central cavity where BPG…

A: Humans have both Hb A and Hb F hemoglobin which are present in adult and fetal life respectively.…

Q: In addition to Oz binding, changes in other chemical conditions can result in hemoglobin changes. pH…

A: pH effects the ability of haemoglobin to carry oxygen. This can be explained with help of Bohr's…

Q: Sickle cell anemia is associated with which of the following:   Question 27 options:   Sticky…

A: Sickle cell anemia : It is caused by the single acidic amino acid substitution, Glu 6th position to…

Q: 10) The saturation curves for two myoglobin mutants are shown below. a) Estimate the K, values for…

A: Hemoglobin molecule of red blood cells binds oxygen. The degree to which the amount of oxygen binds…

Q: Certain antibodies have been shown to bind only to the folded structure oftheir target protein…

A: Protein interact covalently with the specific protein and functional groups. other complementary…

Q: 2.(a) The two diagrams on the right compare O2 binding pro- perties of Hb Kariya, a human hemo-…

A: Here we are given the hill plot for analyzing the binding kinetics of 2 hemoglobin (Hb) variants,…

Q: Below is the reaction catalyzed by carbonic anhydrase. Please answer the following questions. CO₂ +…

A: The carbon dioxide from the tissue diffuses into the RBC where carbonic anhydrase converts it into…

Q: This question has to do with vesicle traffic. (A) What is the complete mechanism for coat assembly…

A: Proteins synthesized through translation are delivered to the target sites through a process known…

Q: Below is the O2 binding curve for adult Hb in whole blood (containing BPG) shown in red (labelled…

A: The oxygen–hemoglobin dissociation curve, also known as the oxyhemoglobin dissociation curve or the…

Q: Monoclonal antibodies can be conjugated to an insoluble support by chemical methods. Explain how…

A: The cloning of immune cells results in the generation of similar immunoglobulins called monoclonal…

Q: Hemoglobin from different species can have different numbers of subunits. Let's consider three…

A: Hemoglobin (Hb) is the oxygen transport protein found in most vertebrates. Hb in nature exhibits…

Q: In an experiment, hemoglobin is dissociated in a buffer and a subunit is isolated to study for its…

A: Introduction Hemoglobin is an oligomeric, allosteric quaternary structure protein consisting of a…

Q: lysozyme was purified through G50 beads. From your lab experience, you can determine that when going…

A: Chromatography is biochemical separation method for organic molecules or solutes of a compound…

Q: Amoeboid cells that migrate through our tissues, such as the class of white blood cells known as…

A: RAC is responsible for protrusion as well as attachment and thus, dominates at the leading edge…

Q: What can be deduced from the following information? % N- C- Protein Nonpolar polar acidic basic term…

A: Polar aminoacids are more. Hence hydrophilicity is more. Globular proteins are folded like…

Q: What would you expect the normal clearance value for amino acids to be? Explain.

A: A pharmacokinetic assessment of the amount of plasma from which a drug is absolutely extracted per…

Q: What is the name of themolecule that transportsoxygen in red blood cells?

A: The red blood cells (RBC) are enucleated cells that transport oxygen molecules from the lungs to…

Q: "Hemoglobin can be in either an oxy- or deoxy- state at a given pH. Given the appropriate pKa…

A: Henderson havelbalch equation describes the derivation of pH as measure of acidity using pKa , the…

Q: In the precipitation of Proteins by heavy metal salts, are the salts completely removed from the…

A: Adding chaotropic substances like the salts, increases the entropy and can thus destabilize the…

Q: 2B. S. aureus hemolysin B attacks the RBC cell membrane by hydrolyzing the sphingomyelin headgroup:…

A: Sphingomyelin is a phospholipid with sphingosine  as platform molecule and phosphocholine as head…

Q: Our current experimental procedure works because Con A adheres strongly to the nitrocellulose…

A: The correct option is C A membrane-binding protein.

Q: If "P-labeled inorganic phosphate were introduced to erythrocytes undergoing glycolysis, would you…

A: Glycolysis is the first step of cellular respiration. This metabolic process serves as the…

Q: How does the difference between the-chain and the -chain of hemoglobin explain the differences…

A: Hemoglobin is an oligomeric, allosteric, conjugated protein with four polypeptide chains joined by…

Q: The 2-3 phosphoglycerate (BFG) binds to the central gap formed by the hemoglobin monomers (a2b2)…

A: The activity of molecule 2,3 phosphoglycerate (BPG) is important to obtain stabilized structure of…

Q: Inside a beaker, dialysis membrane A is filled with a 50% glucose solution and dialysis membrane B…

A: Osmosis is the net movement of water from the area of lower solute concentration to an area of a…

Q: Amoeboid cells that migrate through our tissues, such as the class of white blood cells known as…

A: Cytoskeletal frame work of the cell is responsible for the cell motility, contraction and movement…

Q: (a) (' . Hill plots. Label the axes and indicate on both the plot above and the Hill plot where the…

A: We know that nitrous oxide are  anesthetic gas that are used during surgery  and are non volatile in…

Q: 8. In a patient with anemia, the presence of Heinz bodics in the RBCS was the result of the…

A: