Answered: You are performing an enzymatic…

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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You are performing an enzymatic reaction to digest DNA and nothing is working.

The working solution for the digestion buffer should contain 400 mM Tris, pH 8.0 and 125 mM MgCl2.

Mixed are:

0.2 ml of 2M Tris, pH 8.0 stock solution

0.25 ml of 5M MgCl2 stock solution

*Solution completed with 0.55 ml water to make a final volume of 1ml.

a) Before checking the calculation, what important question do you have to ask first for this specific solution? (not a generic question about all solutions)

b) You are satisfied with the answer to a) and you believe he made a dilution error. You use the equation that applies to dilutions to check his calculations. Aha! you find the problem. What is it and how did you find out?

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