2. 500 ml of a 0.1 M sodium phosphate buffer with pKa = 7.21, is diluted a) How would you dilute this buffer 100 times to make a final volume of 50 ml? Show the ratios to be added together. b) Calculate the pH of the buffer when it is diluted with dH20 c) Calculate the pH of the buffer when it is diluted with 0.3 M KCI 3. What is the normality of a 5 M phosphoric acid (SG = 1.88 g/cm3; 85% solution has a Mr of 98 g/mole %3D

2. 500 ml of a 0.1 M sodium phosphate buffer with pKa = 7.21, is diluted a) How would you dilute this buffer 100 times to make a final volume of 50 ml? Show the ratios to be added together. b) Calculate the pH of the buffer when it is diluted with dH20 c) Calculate the pH of the buffer when it is diluted with 0.3 M KCI 3. What is the normality of a 5 M phosphoric acid (SG = 1.88 g/cm3; 85% solution has a Mr of 98 g/mole %3D

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

See similar textbooks

Similar questions

Calculate the appropriate volume (in µL) of 9X loading buffer that should be added to 46.0 µL of a sample prior to loading the sample on an agarose gel.
explain step by step why the correct answer is C
It takes 175ml of 0.75 M H2SO4 to completely neutralize 285 ml of NaOH. What is the concentration of the NaOH solution?
Refer to the following titration curve below: 13 12 11 10 7 5 4 3 2 8 10 12 14 16 18 20 22 24 26 28 30 Volume of Titrant / mL Unknown Acid 0.10 mol/L - titrant = NaOH 0.1 mol/L How many buffering regions are present in the titration curve of the amino acid?
N-(2-hydroxyethyl)piperazine-N'-(2-ethanesulfonic acid) buffer at pH 7 with 400 mM NaCl. A A purified protein is in a Hepes dialysis membrane tube holds a 5.0 mL sample of the protein solution. The sample tube floats in a beaker containing 0.50 L of the same Hepes buffer, but with 0 mM NaCl, for dialysis. Small molecules and ions (such as Na*, CI", and Hepes) can to diffuse across the dialysis membrane, but the protein cannot. Assume there are no sample volume changes during the dialysis. Calculate the final concentration of NaCl in the protein sample once the dialysis has come to equilibrium. Calculate the final NaCl concentration in the 5.0 mL protein sample after dialysis in 300 mL of the same Hepes buffer, with 0 mM NaCl, twice in succession. [NaCl] after a single dialysis: [NaCl] after a double dialysis: mM mM
50 g of dextrose is added to1 L of SWFI. What is the final concentration?
3. a) An aqueous solution is 3.50% by mass potassium bromide, KBr, and has a density of 1.02 g/mL. The molality of potassium bromide in the solution is _________m. b)An aqueous solution of magnesium nitrate has a concentration of 0.474 molal. The percent by mass of magnesium nitrate in the solution is _________%. c)An aqueous solution of cobalt(II) bromide has a concentration of 0.153 molal. The percent by mass of cobalt(II) bromide in the solution is _________%.
Calculate the mole ratio of sodium acetate and acetic acid needed to make a buffer of p H 4.68 ( see Table 15.1 for Ka values or Appendices G and H for Ka and Ksp values respectively.