1. (a) Perform the calculations required to prepare 100ml of 0.20M sodium acetate buffer of pH 5.40 if you are given a bottle of sodium acetate (M.W = 82) and a bottle of concentrated hydrochloric acid (Normality = 11.M). The pKa of acetic acid is 4.80. (b) After the calculations, prepare the buffer. Check the pH of your reparation and comment on your results. 3. Prepare 1:1, 1:4, 1:10, and 1:100 dilutions of your buffer and obtain pH readings for them. Plot on a graph paper pH against buffer concentration. Explain the shape of the graph.
1. (a) Perform the calculations required to prepare 100ml of 0.20M sodium acetate buffer of pH 5.40 if you are given a bottle of sodium acetate (M.W = 82) and a bottle of concentrated hydrochloric acid (Normality = 11.M). The pKa of acetic acid is 4.80. (b) After the calculations, prepare the buffer. Check the pH of your reparation and comment on your results. 3. Prepare 1:1, 1:4, 1:10, and 1:100 dilutions of your buffer and obtain pH readings for them. Plot on a graph paper pH against buffer concentration. Explain the shape of the graph.
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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1. (a) Perform the calculations required to prepare 100ml of 0.20M sodium acetate buffer of pH 5.40 if you are given a bottle of sodium acetate (M.W = 82) and a bottle of concentrated hydrochloric acid (Normality = 11.M). The pKa of acetic acid is 4.80.
(b) After the calculations, prepare the buffer. Check the pH of your reparation and comment on your results. 3. Prepare 1:1, 1:4, 1:10, and 1:100 dilutions of your buffer and obtain pH readings for them.
Plot on a graph paper pH against buffer concentration. Explain the shape of the graph.
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