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pH Dilution Factor 3.5 5.5 7.5 5 5 50 Absorbance 0.098 0.068 0.028 0.032 Conc.(mg/ml) 0.195 0.042 0.130 0.044 0.053 4.179% 0.9% 2.786% 9.429% 11.357% % solubility The above table indicates the concentration of protein in the diluted supernatant and the supernatant before dilution at different pH. Protein Solubility Versus pl 4.5 5 0.027 7.5 6.5 8.5 50 0.054 0.100 21.429% pll Value The above figure shows a plot of protein solubility versus pH. Please provide a brief discussion and explanation of the results. (using isoelectric point and net charge to explain)

pH Dilution Factor 3.5 5.5 7.5 5 5 50 Absorbance 0.098 0.068 0.028 0.032 Conc.(mg/ml) 0.195 0.042 0.130 0.044 0.053 4.179% 0.9% 2.786% 9.429% 11.357% % solubility The above table indicates the concentration of protein in the diluted supernatant and the supernatant before dilution at different pH. Protein Solubility Versus pl 4.5 5 0.027 7.5 6.5 8.5 50 0.054 0.100 21.429% pll Value The above figure shows a plot of protein solubility versus pH. Please provide a brief discussion and explanation of the results. (using isoelectric point and net charge to explain)

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Protein Solubility 3.5 5.5 5 5 0.068 0.028 Absorbance 0.098 Conc.(mg/ml) 0.195 0.130 0.044 0.9% 2.786% 9.429% 11.3: % solubility 4.179% The above table indicates the concentration of protein in the diluted supernatant and the supernatant before dilution at different pH. Dilution Factor 30 22.5 15 PH 7.5 1.5 4.5 5 0.027 3 0.042 Protein Solubility Versus pH 6.5 6 4.5 pH Value The above figure shows a plot of protein solubility versus pH. 50 7.5 7.5 50 0.032 0.053 8.5 50 0.054 0.100 21.429% 9 Please provide a brief discussion and explanation of the results. (using isoelectric point and net charge to explain)
Transcribed Image Text:Protein Solubility 3.5 5.5 5 5 0.068 0.028 Absorbance 0.098 Conc.(mg/ml) 0.195 0.130 0.044 0.9% 2.786% 9.429% 11.3: % solubility 4.179% The above table indicates the concentration of protein in the diluted supernatant and the supernatant before dilution at different pH. Dilution Factor 30 22.5 15 PH 7.5 1.5 4.5 5 0.027 3 0.042 Protein Solubility Versus pH 6.5 6 4.5 pH Value The above figure shows a plot of protein solubility versus pH. 50 7.5 7.5 50 0.032 0.053 8.5 50 0.054 0.100 21.429% 9 Please provide a brief discussion and explanation of the results. (using isoelectric point and net charge to explain)
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