Formula for % solubility of proteins: % solubility = [(C x V x F) / (1000 x 35%)] x 100% C = concentration (mg/ml); F = dilution factor; V = volume of solution (ml) Calculate the % solubility of protein in the soy flour (Assume the soy flour contains 35% protein (w/w))

Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
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pH

3.5

4.5

5.5

6.5

7.5

8.5

Absorbance

0.098

0.027

0.068

0.028

0.032

0.054

Concentration in diluted supernatant

(mg/ml)

0.196

0.054

0.136

0.0056

0.0064

0.0108

Concentration in undiluted supernatant

(mg/ml)

0.98

0.27

0.68

0.28

0.32

0.54

% solubility

 

 

 

 

 

 

Formula for % solubility of proteins:

% solubility = [(C x V x F) / (1000 x 35%)] x 100%

C = concentration (mg/ml); F = dilution factor; V = volume of solution (ml)

Calculate the % solubility of protein in the soy flour (Assume the soy flour contains 35% protein (w/w))

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