1. How would you make 10 L of 0.3 M citrate buffer pH 2.5 from solutions of 0.4 M NaCitrate and 0.4 M citric acid?  Include the amounts of all components added in mL.  pKa = 3.09 2. How many grams of disodium hydrogen phosphate (142 g/mol), mL of 0.5 M HCl or NaOH, and mL of water are needed to make 500 mL of 0.15 M phosphate buffer pH 11.90? What would be the pH of this solution if it were diluted to 2 L with pure water?  pKa(1) = 2.15; pKa(2) = 6.86; pKa(3) = 12.38 3. How many mL of 3 M NaOH or 3 M HCl and mL of 0.5 M acetate buffer pH 3.78 do you need to make 600 mL of a 0.02 M acetate buffer pH 5.3?

Biochemistry
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ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
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1. How would you make 10 L of 0.3 M citrate buffer pH 2.5 from solutions of 0.4 M NaCitrate and 0.4 M citric acid?  Include the amounts of all components added in mL.  pKa = 3.09

2. How many grams of disodium hydrogen phosphate (142 g/mol), mL of 0.5 M HCl or NaOH, and mL of water are needed to make 500 mL of 0.15 M phosphate buffer pH 11.90? What would be the pH of this solution if it were diluted to 2 L with pure water?  pKa(1) = 2.15; pKa(2) = 6.86; pKa(3) = 12.38

3. How many mL of 3 M NaOH or 3 M HCl and mL of 0.5 M acetate buffer pH 3.78 do you need to make 600 mL of a 0.02 M acetate buffer pH 5.3?

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