1. How would you make 10 L of 0.3 M citrate buffer pH 2.5 from solutions of 0.4 M NaCitrate and 0.4 M citric acid? Include the amounts of all components added in mL. pKa = 3.09 2. How many grams of disodium hydrogen phosphate (142 g/mol), mL of 0.5 M HCl or NaOH, and mL of water are needed to make 500 mL of 0.15 M phosphate buffer pH 11.90? What would be the pH of this solution if it were diluted to 2 L with pure water? pKa(1) = 2.15; pKa(2) = 6.86; pKa(3) = 12.38 3. How many mL of 3 M NaOH or 3 M HCl and mL of 0.5 M acetate buffer pH 3.78 do you need to make 600 mL of a 0.02 M acetate buffer pH 5.3?

1. How would you make 10 L of 0.3 M citrate buffer pH 2.5 from solutions of 0.4 M NaCitrate and 0.4 M citric acid? Include the amounts of all components added in mL. pKa = 3.09 2. How many grams of disodium hydrogen phosphate (142 g/mol), mL of 0.5 M HCl or NaOH, and mL of water are needed to make 500 mL of 0.15 M phosphate buffer pH 11.90? What would be the pH of this solution if it were diluted to 2 L with pure water? pKa(1) = 2.15; pKa(2) = 6.86; pKa(3) = 12.38 3. How many mL of 3 M NaOH or 3 M HCl and mL of 0.5 M acetate buffer pH 3.78 do you need to make 600 mL of a 0.02 M acetate buffer pH 5.3?

Basic Clinical Laboratory Techniques 6E

6th Edition

ISBN:9781133893943

Author:ESTRIDGE

Publisher:ESTRIDGE

Chapter1: The Clinical Laboratory

Section1.8: Laboratory Math And Reagent Preparation

Problem 12RQ

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