Provided Stock solution of Coomassie Blue: 0.500 mg/mL Volume of 0.500 mg/ml Solution Dilution to ID be made A D E F 1/5 of 0.500 mg/mL stock 1/8 of Solution A 4/5 of Solution B 3/5 of Solution B 2/5 of Solution B 1/5 of Solution B [Coomassie Blue] (mg/mL) Required Total volume 10.0 mL 10.0 mL 2.50 mL 2.50 mL 2.50 mL 2.50 mL Coomassie Blue (mL) 1 -- 1 Volume of Solution A (ml) Volume of Solution B (mL) 1 Volume of water (mL)
Provided Stock solution of Coomassie Blue: 0.500 mg/mL Volume of 0.500 mg/ml Solution Dilution to ID be made A D E F 1/5 of 0.500 mg/mL stock 1/8 of Solution A 4/5 of Solution B 3/5 of Solution B 2/5 of Solution B 1/5 of Solution B [Coomassie Blue] (mg/mL) Required Total volume 10.0 mL 10.0 mL 2.50 mL 2.50 mL 2.50 mL 2.50 mL Coomassie Blue (mL) 1 -- 1 Volume of Solution A (ml) Volume of Solution B (mL) 1 Volume of water (mL)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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fill in the volumes required to make the various dilutions of Coomassie Blue and their respective concentrations. show sample calculations.
![Provided Stock solution of Coomassie Blue: 0.500 mg/mL
Volume of
0.500 mg/ml
Solution Dilution to
ID
be made
A
B
C
D
E
F
1/5 of
0.500 mg/mL
stock
1/8 of
Solution A
4/5 of
Solution B
3/5 of
Solution B
2/5 of
Solution B
1/5 of
Solution B
[Coomassie
Blue]
(mg/mL)
Required
Total
volume
10.0 mL
10.0 mL
2.50 mL
2.50 mL
2.50 mL
2.50 mL
Coomassie
Blue (ml)
1
1
1
1
Volume
of
Solution
A (mL)
1
1
1
1
Volume of
Solution B
(mL)
Volume
of water
(mL)](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F1fabc3ca-e22c-4a82-92cc-264bbcbec85e%2F355b3c57-706f-4482-9f64-f56d78216b2f%2Fj4x5vk_processed.png&w=3840&q=75)
Transcribed Image Text:Provided Stock solution of Coomassie Blue: 0.500 mg/mL
Volume of
0.500 mg/ml
Solution Dilution to
ID
be made
A
B
C
D
E
F
1/5 of
0.500 mg/mL
stock
1/8 of
Solution A
4/5 of
Solution B
3/5 of
Solution B
2/5 of
Solution B
1/5 of
Solution B
[Coomassie
Blue]
(mg/mL)
Required
Total
volume
10.0 mL
10.0 mL
2.50 mL
2.50 mL
2.50 mL
2.50 mL
Coomassie
Blue (ml)
1
1
1
1
Volume
of
Solution
A (mL)
1
1
1
1
Volume of
Solution B
(mL)
Volume
of water
(mL)
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