a. calculate the volume of 0.1 M H2PO4– required to prepare 100 mL of your assigned buffer. b. calculate the volume  of 0.1 M HPO42– required to prepare 100 mL of your assigned buffer.  1. Assigned pH Value   ___6.90_________ 2. pKa Value of Assigned Buffer   ___7.20_________ 3. Ratio 0.5011

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Chapter1: Biochemistry: An Evolving Science
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a. calculate the volume of 0.1 M H2PO4 required to prepare 100 mL of your assigned buffer.

b. calculate the volume  of 0.1 M HPO42– required to prepare 100 mL of your assigned buffer. 

1. Assigned pH Value

  ___6.90_________

2. pKa Value of Assigned Buffer

  ___7.20_________

3. Ratio

0.5011

Expert Solution
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A buffer solution is an aqueous solution consisting of a mixture of weak acid and it's conjugate base, or vice versa. It maintains the pH of solution on addition of small amount of acid or base by absorbing added H= ions or OH- ions into its reserve acidity or basicity.

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