- You are given a buffer that is labeled 10x and your instructor asks you to dilute it to 1x in the final tube. If you have a final volume of 100uL in your tube how much of the 10x buffer will you need to add to bring it to 1x? Do you need to add anything else to the tube before using the new solution?
Introduction:
For incorporating more solvents, dilution is the process of lowering a solute's concentration in a solution. Compared to the initial solution, the final solution has a reduced solute concentration. Dilution is a popular method used in many disciplines, including chemistry, biology, and medicine, to modify a solution's strength to the ideal level for a given application. In a dilution, the quantity of solute molecules stays constant, but as the volume of the solution rises, the solute concentration falls. The formula C1 x V1 = C2 x V2, where C1 is the starting concentration, V1 is the initial volume, C2 is the final concentration, and V2 is the final volume, can be used to determine the solute concentration in a diluted solution.
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