I am trying to figure out how much albumin and globulin are in our egg white sample. The egg white is 38.51g. It was diluted with 200 mL of water, and I was told to find the dilution factor. We were told that 38.5g is equal to 1mL of water. The dilution factor I calculated was 38.51mL (egg white)/238.51mL (total volume)= 1:6 dilution factor. We then took 40mL of the diluted sample and centrifuged it to separate the albumin and globulin. Using a given standard curve, we calculated the concentrations of albumin and globulin for each tube with different amounts of each protein. There were other solutions added to each tube for the spectrophotometer, but each one had a total volume of 5mL including the albumin and globulin. To find mg protein in the tube, do I multiply the concentration by the total volume in the tube or by the mL of protein added to each tube? To calculate the amount of each protein in the egg white, I multiplied each concentration in the table by the dilution factor, 6.19. But I do not know what to do after that. Tube mL albumin mL globulin mg protein/mL (from standard curve) mg protein in tube 1 0.2 0 3.19 2 0.4 0 3.99 3 0 1.0 0.79 4 0 1.2 1.77
Molecular Techniques
Molecular techniques are methods employed in molecular biology, genetics, biochemistry, and biophysics to manipulate and analyze nucleic acids (deoxyribonucleic acid (DNA) and ribonucleic acid (RNA)), protein, and lipids. Techniques in molecular biology are employed to investigate the molecular basis for biological activity. These techniques are used to analyze cellular properties, structures, and chemical reactions, with a focus on how certain molecules regulate cellular reactions and growth.
DNA Fingerprinting and Gel Electrophoresis
The genetic makeup of living organisms is shown by a technique known as DNA fingerprinting. The difference is the satellite region of DNA is shown by this process. Alex Jeffreys has invented the process of DNA fingerprinting in 1985. Any biological samples such as blood, hair, saliva, semen can be used for DNA fingerprinting. DNA fingerprinting is also known as DNA profiling or molecular fingerprinting.
Molecular Markers
A known DNA sequence or gene sequence is present on a chromosome, and it is associated with a specific trait or character. It is mainly used as a genetic marker of the molecular marker. The first genetic map was done in a fruit fly, using genes as the first marker. In two categories, molecular markers are classified, classical marker and a DNA marker. A molecular marker is also known as a genetic marker.
DNA Sequencing
The most important feature of DNA (deoxyribonucleic acid) molecules are nucleotide sequences and the identification of genes and their activities. This the reason why scientists have been working to determine the sequences of pieces of DNA covered under the genomic field. The primary objective of the Human Genome Project was to determine the nucleotide sequence of the entire human nuclear genome. DNA sequencing selectively eliminates the introns leading to only exome sequencing that allows proteins coding.
I am trying to figure out how much albumin and globulin are in our egg white sample.
The egg white is 38.51g. It was diluted with 200 mL of water, and I was told to find the dilution factor. We were told that 38.5g is equal to 1mL of water. The dilution factor I calculated was 38.51mL (egg white)/238.51mL (total volume)= 1:6 dilution factor.
We then took 40mL of the diluted sample and centrifuged it to separate the albumin and globulin.
Using a given standard curve, we calculated the concentrations of albumin and globulin for each tube with different amounts of each protein. There were other solutions added to each tube for the spectrophotometer, but each one had a total volume of 5mL including the albumin and globulin.
To find mg protein in the tube, do I multiply the concentration by the total volume in the tube or by the mL of protein added to each tube?
To calculate the amount of each protein in the egg white, I multiplied each concentration in the table by the dilution factor, 6.19. But I do not know what to do after that.
| Tube | mL albumin | mL globulin | mg protein/mL (from standard curve) | mg protein in tube |
| 1 | 0.2 | 0 | 3.19 | |
| 2 | 0.4 | 0 | 3.99 | |
| 3 | 0 | 1.0 | 0.79 | |
| 4 | 0 | 1.2 | 1.77 |
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