make a schematic diagram of Iodine value determination using this data. Write the schematic diagram of lodine Value Determination as shown in the video by AmritaVlab:Pipette out 10 ml of fat sample in chloroform into an iodination flaskand label it Test' and stopper the flask. Pipette out 20 ml of lodineMonochloride Reagent into the flask and label it 'Test' and stopper theiodination flask and then swirl the flask to mix the contents. Kept theflask in the dark for incubation at a room temperature for 30 minutes.Prepare a blank sample by pipetting a 10 ml chloroform in aniodination flask and label it as 'Blank'. Pipette out 20ml lodineMonochloride reagent to the flask labeled 'Blank', and stopper theiodination flask and swirl the flask go mix the contents. The flasklabeled 'Blank' is then kept in dark for incubation at room temperaturefor 30 minutes. After 30 minutes of incubation, take out the flasklabeled 'Test'.Pipette out 10 ml potassium iodide solution into the flask labeledTEST. Stopper the flask and gently swirl it to mix the contents well.The color of the solution changes to dark red brown. Rinse the flaskwith 30 ml distilled water and mix the contents again. The contentsof the flask labeled 'Test' is then titrated against 0.1N sodiumthiosulphate.Continue the titration till the solution turns to pale straw colour. Add 1ml of starch solution into the flask. The color of the solution changesto purple blue. The disappearance of the blue colour is recorded asthe end point of the titrationThe procedure is repeated for the flask labeled 'Blank'. Pipette out10 ml potassium iodide solution into the Blank' after 30 minutesincubation. Swirl it to mix the contents well. The color of the solutionchanges to dark red brown. Rinse with 30 ml distilled water and mixthe contents again. The contents of the flask labeled 'Blank' are thentitrated against 0.1N sodium thiosulphate.Continue the titration till the solution turns to pale straw colour.Add 1 ml of starch solution into the flask. The color of the solutionchanges to purple blue. Titration is continued until the color of thesolution in the flask turns colourless. The disappearance of theblue color is recorded as the end point of the titration.The volume of Sodium thiosulphate used is obtained from the titrevalues ((Blank- Test). The lodine value can be calculated using theformula: lodine value = Equivalent Weight of lodine * (volume ofsodium thiosulphate used) * Normality of sodium thiosulphate* 100*10-3 Weight of fat sample used for analysis (g]. Where EquivalentWeight of lodine is 127 and normality of sodium thiosulphate is 0.1.

Simple fats are triglycerides. Triglycerides or triacylglycerols are fatty acid esters of glycerol.…

Answered: Write the schematic diagram of lodine…

Science

Biochemistry

Write the schematic diagram of lodine Value Determination as shown in the video by Amrita Vlab:

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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