A newly developed qPCR has an efficiency of 75%, and each cycle is pretty consistent. In this qPCR, if a reporter and quencher located at the ends of a probe, respectively, are separated far enough from each other, one (1) probe produces a signal of S. Let's assume that we start with only one (1) copy of target dsDNA. How much signals can we obtain after 15 cycles in terms of S if we use the following prob
A newly developed qPCR has an efficiency of 75%, and each cycle is pretty consistent. In this qPCR, if a reporter and quencher located at the ends of a probe, respectively, are separated far enough from each other, one (1) probe produces a signal of S. Let's assume that we start with only one (1) copy of target dsDNA. How much signals can we obtain after 15 cycles in terms of S if we use the following prob
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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A newly developed qPCR has an efficiency of 75%, and each cycle is pretty consistent. In this qPCR, if a reporter and quencher located at the ends of a probe, respectively, are separated far enough from each other, one (1) probe produces a signal of S. Let's assume that we start with only one (1) copy of target dsDNA. How much signals can we obtain after 15 cycles in terms of S if we use the following probes?
(a) Molecular beacon probe
(b) TaqMan probe
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Hello, I still can't understand why we get about 24500 signals. Please let me know what formula I should use.
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