15) After purification of the enzyme Mediculase from a blood sample, you tried to calculate different enzyme parameters. Provided the saturated enzyme activity is 15 umol / min and you have 1.5 ug of the enzyme which has a molecular weight of 10,000 g/mol. The Michalis constant of the enzyme is (assume the association rate constant for (E+S) is (4x1043 /micro M.Min) and the dissociation rate constant for the ES complex into E+S is (3x1045 / Min)). Select one: A) 0.001 MICRO M B) 1 MICROM C) 100 MICRO M D) 0.010 MICRO M E) Cannot be calculated from the given information. Answer: C
15) After purification of the enzyme Mediculase from a blood sample, you tried to calculate different enzyme parameters. Provided the saturated enzyme activity is 15 umol / min and you have 1.5 ug of the enzyme which has a molecular weight of 10,000 g/mol. The Michalis constant of the enzyme is (assume the association rate constant for (E+S) is (4x1043 /micro M.Min) and the dissociation rate constant for the ES complex into E+S is (3x1045 / Min)). Select one: A) 0.001 MICRO M B) 1 MICROM C) 100 MICRO M D) 0.010 MICRO M E) Cannot be calculated from the given information. Answer: C
Biochemistry
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ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
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Transcribed Image Text:15) After purification of the enzyme Mediculase from a blood sample, you tried to calculate different enzyme
parameters. Provided the saturated enzyme activity is 15 umol / min and you have 1.5 ug of the enzyme which has a
molecular weight of 10,000 g/mol. The Michalis constant of the enzyme is (assume the association rate constant for
(E+S) is (4x1043 /micro M.Min) and the dissociation rate constant for the ES complex into E+S is (3x1045 / Min)).
Select one:
A) 0.001 MICRO M
B) 1 MICROM
C) 100 MICRO M
D) 0.010 MICRO M
E) Cannot be calculated from the given information.
Answer: C
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