Materials - 2021FA-CHM-1O XBb 4427577d-fleet02-xythos.content.blackboardcdn.com/6086c260d7e8f/4427577?X-Blackboard-Expiration=1633143600000&X-Blackboard-Sig6 / 9100%Exercise 1: Standard Curve for Protein Measurements:A standard curve for protein concentration is often created using known concentrations of bovine serum albumin (protein).This process is called the Bradford Assay; it is a colorimetric assay. A special reagent turns blue when it binds to aminoacids present in protein. The intensity of the color is best measured with a spectrophotometer (a device for comparing twolight radiations, wavelength by wavelength). In the case of the Bradford Assay the greater the absorbance, the higher theprotein concentration.A series of tests were performed on some samples andspectrophotometer:following measurements were obtained using aProteinConcentration(mg/ml)Absorbance (A)0.260.0980.560.2130.3830.841.120.4731.400.527TASKS:1.Enter the data into Excel - the protein concentration is the independent variable.2. Create a scatter graph of the data that is appropriately titled and labeled (see Introduction).3. Use the Trendline feature of Excel to find the best linear fit to the data.4. Display the equation and the R? value of the trendline on the graph and print out the graph.5. Print the graph, scaling so that it fits on an 8% x 11 in. sheet.Use the equation for the standard curve to determine the protein concentrations of the following unknown samples:6.Absorbance (A)0.0230.2010.6420.760WETOSHIBAF5F6F7F8F9F10F11F12INS

Answer. Bradford method is based on a blue dye known as Coomassie Brilliant Blue that binds to free…

1.40 0.527 ASKS: 1. Enter the data into Excel - the protein concentration is the independent variable. 2. Create a scatter graph of the data that is appropriately titled and labeled (see Introduction). 3. Use the Trendline feature of Excel to find the best linear fit to the data. 4. Display the equation and the R? value of the trendline on the graph and print out the graph. 5. Print the graph, scaling so that it fits on an 8% x 11 in. sheet. 6. Use the equation for the standard curve to determine the protein concentrations of the following unknown samples: Absorbance (A) 0.023 0.201 0.642 0 760

1.40 0.527 ASKS: 1. Enter the data into Excel - the protein concentration is the independent variable. 2. Create a scatter graph of the data that is appropriately titled and labeled (see Introduction). 3. Use the Trendline feature of Excel to find the best linear fit to the data. 4. Display the equation and the R? value of the trendline on the graph and print out the graph. 5. Print the graph, scaling so that it fits on an 8% x 11 in. sheet. 6. Use the equation for the standard curve to determine the protein concentrations of the following unknown samples: Absorbance (A) 0.023 0.201 0.642 0 760

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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