1. In a serial dilution, one initially sets a starting dilution and adds an aliquot portion of itsuccessively to other tubes. Refer to the table below and fill in the missing values.Tube NumberTube 1ContentsAfterTube Discard1 mLTubeTubeStartingdilutiontransferring1 mL to Tube 24Serum (mL)0.20.1Diluent (mL)1.80.9Final total volume (mL):after transferring 1 mL tothe next tube2DilutionVolume of serum1:10Total volume of solutionDilution factor(represents thereciprocal of dilution)Note: In dilution, the volume of serum decreases, while the volume of diluent increases.Therefore, the antibody concentration decreases as well.10

Serial Dilution is a important procedure to obtain the desired concentration of reagent or serum or…

Answered: 1. In a serial dilution, one initially…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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1. In a serial dilution, one initially sets a starting dilution and adds an aliquot portion of it successively to other tubes. Refer to the table below and fill in the missing values.