1. In a serial dilution, one initially sets a starting dilution and adds an aliquot portion of itsuccessively to other tubes. Refer to the table below and fill in the missing values.Tube NumberTube 1ContentsAfterTube Discard1 mLTubeTubeStartingdilutiontransferring1 mL to Tube 24Serum (mL)0.20.1Diluent (mL)1.80.9Final total volume (mL):after transferring 1 mL tothe next tube2DilutionVolume of serum1:10Total volume of solutionDilution factor(represents thereciprocal of dilution)Note: In dilution, the volume of serum decreases, while the volume of diluent increases.Therefore, the antibody concentration decreases as well.10

Serial Dilution is a important procedure to obtain the desired concentration of reagent or serum or…

Answered: 1. In a serial dilution, one initially…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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(e) Complete the following table: Number printed on the micropipette's end (its plunger) 20 20 200 1000 1000 Number showing in the micropipette's display window OTOHOSHOSHOOO 0 7 0 1 0 5 1 0 5 1 0 0 0 2 1 Volume that the micropipette is set to draw up (include units in your answer) 6) Before using a micropipette, you have to first place a 7) Shown below are the steps required to transfer fluid from one tube to another using a micropipette. Write numbers in the blanks to indicate the correct order in which the steps should be performed. Slowly release the micropipette's plunger. Place the micropipette tip into the tube containing fluid you wish to draw up. Push down on the micropipette plunger until it reaches the second stop. Place the micropipette tip into the tube that you wish to expel fluid into. Push down on the micropipette plunger until it reaches the first stop. 8) Suppose that you're getting ready to draw fluid into a micropipette. To do so, you push the micropipette's plunger…
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1. In a serial dilution, one initially sets a starting dilution and adds an aliquot portion of it successively to other tubes. Refer to the table below and fill in the missing values.