Based on your observations recorded in the previous question, list the possible genotype(s) of strains 1, 2, and 3, if each strain only had one mutation within its/lac operon 3. Strain Possible Genotype(s) 1 2 3
Based on your observations recorded in the previous question, list the possible genotype(s) of strains 1, 2, and 3, if each strain only had one mutation within its/lac operon 3. Strain Possible Genotype(s) 1 2 3
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Using question 2 you can answer question 3 which I need help with

Transcribed Image Text:3. Based on your observations recorded in the previous question, list the possible genotype(s) of
strains 1, 2, and 3, if each strain only had one mutation within its/lac operon.
Strain
Possible Genotype(s)
1
3.

Transcribed Image Text:2. Complete the following table based on your B-galactosidase assay.
Susstrute
Mellow
Color Change
E. coli strain
IPTG
Water
ONPG
buffer
Observation
A
0.3 ml wild-type
0.4 ml
100 µl
10 µl
Nettow
0.3 ml wild-type
0.4 ml
100 µl
10 µl
0.3 ml strain 1
0.4 ml
100 µl
10 µl
Yelloce
C
0.3 ml strain 1
0.4 ml
100 µl
10μ
yellow.
E
0.3 ml strain 2
0.4 ml
100 µl
10 µl
Clear
F
0.3 ml strain 2
0.4 ml
100 µl
10 ul
Clear
0.3 ml strain 3
0.4 ml
100 ul
10 µl
0.3 ml strain 3
0.4 ml
100 µl
10 ul
0.3 ml negative
0.4 ml
100 µl
10 µl
control (no E. coli)
0.3 ml negative
0.4 ml
100 ul
10 µl
control (no E. coli)
Expert Solution

Step 1
test detects the presence or absence of Galactosidase enzyme in the bacterial strain. This enzyme. This enzyme is necessary for lactose metabolism in bacteria.
Step by step
Solved in 3 steps

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