A phenylgalactoside-type compound, which yields galactose when enzymatically cleaved, was added as the sole carbon source to petri dishes containing E. coli culture medium. E. coli can utilize galactose for growth. Each of the following bacterial strains was plated on this medium and scored for growth after 1-2 days. Growth Genotype lacZ+ lacI+ lacz+ lacIs - lacz+ lacl- oc lacZ+ lacI+ + a) What do you conclude about this compound with respect to its ability to be cleaved by ß- galactosidase? Why? b) What do you conclude about this compound with respect to its ability to inactivate the Lac repressor? Why? +

Q: Why is GAPDH not be a suitable loading control for nuclear extracts?

A: * In many molecular biology assays, such as Western blotting, the housekeeping gene glyceraldehyde…

Q: What medium is this? What is it detecting? Why do you see the clear ring around one bacteria (how…

A: Caesin Hydrolysis Test This test is used to determine the ability of the provided bacteria to…

Q: Give two reasons as to why anyone would bother SYBRGreen (SG) staining samples that contain…

A: SYBR Green (SG) is an asymmetrical cyanine nucleic acid stain. It binds to DNA.

Q: Lucas's unwashed, dirty hand produces 947 CFU when swabbed onto a nutrient agar plate, while his…

A: CFU stands for colony forming unit.

Q: Does Escherichia coli provide positive or negative results for the lysine decarboxylase and…

A: Ornithine decarboxylase test detects whether bacteria uses ornithine amino acid for carbon source…

Q: Based on the same two strains of E. coli from the previous question, why do you see growth in the…

A: Ampicillin is a penicillin type of antibiotic substances containing medium So the  strain II can…

Q: E. coli has a doubling time of 0.345 hours at 37 0C. Starting with 1 milligram of cells, assuming a…

A: Microorganisms have complex interactions with their environment and with other microorganisms, and…

Q: Menaquinones is produced by Flavobacterium meningosepticum via submerged fermentation with 60%…

A: Menaquinone are commonly known as vitamin K2. The structure of compound indicates that the compound…

Q: in a clean, non-sterile 15 mL centrifuge tube, prepare a 2.0% yeast suspension by adding 0.06 g…

A: Introduction  Dilution is the process of reducing the concentration of a solution by adding a…

Q: What is the purpose of the negative and positive control in the Disc diffusion assay? Why is it…

A: The disc diffusion assay is used to determine the susceptibility of test bacteria to different…

Q: Explain why the growth of both the strains A and C in medium III is not similar to that of strain B.…

A: Answer: Introduction: In most situations, glucose is the good carbon source for E. coli: it gives…

Q: Could any one provide me with the protocol for Plasma Membrane Isolation via sucrose density…

A: Ultracentrifugation is the technique of high-speed centrifugation in which intracellular components…

Q: In the Avery, McLeod, McCarty Experiment where supernatant from heat killed, virulent S Strain…

A: In this talk, we will learn about a very important experiment called the Avery, McLeod, and McCarty…

Q: The following pictures show the results of a Disk Diffusion Assay for different types of bacteria.…

A: Disk diffusion assay is a laboratory test used to determine the effectiveness of antimicrobial…

Q: A 3.0 L batch bioreactor is used to grow E. coli bacteria, which have an initial concentration of…

A: A batch bioreactor is a closed device utilized to grow microorganisms for example bacteria, yeast,…

Q: What is the genus and species of your bacteria? And What led you to confirm this result and why?…

A: My bacteria is Alcaligenes faecalis :  Genus of Alcaligenes faecalis :Alcaligenes Alcaligenes is a…

Q: A bacterial culture was grown for 12 hours. At 4-hour interval, the culture was sampled to determine…

A: To keep the number of colonies on the plate within a certain range, serial dilutions of culture are…

Gene Interactions

When the expression of a single trait is influenced by two or more different non-allelic genes, it is termed as genetic interaction. According to Mendel's law of inheritance, each gene functions in its own way and does not depend on the function of another gene, i.e., a single gene controls each of seven characteristics considered, but the complex contribution of many different genes determine many traits of an organism.

Gene Expression

Gene expression is a process by which the instructions present in deoxyribonucleic acid (DNA) are converted into useful molecules such as proteins, and functional messenger ribonucleic (mRNA) molecules in the case of non-protein-coding genes.