Choose the statement that best describes the outcome of transfecting cells with a plasmid that includes the genetic promoter of the hypothetical X protein in a plasmid coding for GFP. A.GFP will be expressed under the same conditions and in the same cells as the X protein. B.GFP combined with the X protein will be expressed in all transfected cells. C.PFM will not be expressed as it requires its own sponsor, the PFM sponsor, to be properly expressed. D.GFP will replace the X protein in transfected cells.
Bacterial Genomics
The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.
Transformation Experiment in Bacteria
In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.
Plasmids and Vectors
The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.
Choose the statement that best describes the outcome of transfecting cells with a plasmid that includes the genetic promoter of the hypothetical X protein in a plasmid coding for GFP.
A.GFP will be expressed under the same conditions and in the same cells as the X protein.
B.GFP combined with the X protein will be expressed in all transfected cells.
C.PFM will not be expressed as it requires its own sponsor, the PFM sponsor, to be properly expressed.
D.GFP will replace the X protein in transfected cells.
A plasmid is an extra chromosomal DNA present within a cell. It is physically separated from the chromosomal DNA of an organism. It can replicate independently. It is commonly found in bacteria.
Transfection is the process by which molecular biologist artificially introduce nucleic acids (DNA or RNA) into the target organisms by using viruses.
GFP or green fluorescent proteins are the proteins which exhibit green light when exposed to a particular wavelength. In molecular biology, the GFP is used as a reported for the expression of a particular gene.
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