What strategy does a genetically encoded calcium indicator look like to allow fluorescence imaging of only one cell type in an acute slice of the brain? A.The use of fluorescent protein expression inhibitors in other cells B.The injection of a recombinant virus causing the death of other cells C.The use of a promoter specific to these cells D.Activation of membrane receptors specific to these cells
What strategy does a genetically encoded calcium indicator look like to allow fluorescence imaging of only one cell type in an acute slice of the brain? A.The use of fluorescent protein expression inhibitors in other cells B.The injection of a recombinant virus causing the death of other cells C.The use of a promoter specific to these cells D.Activation of membrane receptors specific to these cells
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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What strategy does a genetically encoded calcium indicator look like to allow fluorescence imaging of only one cell type in an acute slice of the brain?
A.The use of fluorescent protein expression inhibitors in other cells
B.The injection of a recombinant virus causing the death of other cells
C.The use of a promoter specific to these cells
D.Activation of membrane receptors specific to these cells
Expert Solution
Step 1
- A crucial part of astrocyte physiology and function is Ca2+ signaling.
- Ca2+ imaging is the most widely used approach for studying glial cell physiology.
- While chemical Ca2+ indicators have been used for decades to view and monitor changes in glial cell cytosolic Ca2+ concentration, genetically encoded Ca2+ indicators (GECIs) have recently become the gold standard.
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