Which of these statements is correct regarding the CRISPR/Cas9 system in eukaryotic cells? a.Single guide RNA (sgRNA) is used to target specific DNA sequences and activate Cas. b.Bacteriophages are used to inject viral genes into the cell to activated the Cas protein. c.CRISPR takes advantage of the cell’s DNA polymerase proof-reading activities for DNA replication errors d.The CRISPR-Cas system can only be used to alter genomes in prokaryotic cells. e.Cas cuts directly into palindromic restriction sites that it targets in the DNA
Gene Interactions
When the expression of a single trait is influenced by two or more different non-allelic genes, it is termed as genetic interaction. According to Mendel's law of inheritance, each gene functions in its own way and does not depend on the function of another gene, i.e., a single gene controls each of seven characteristics considered, but the complex contribution of many different genes determine many traits of an organism.
Gene Expression
Gene expression is a process by which the instructions present in deoxyribonucleic acid (DNA) are converted into useful molecules such as proteins, and functional messenger ribonucleic (mRNA) molecules in the case of non-protein-coding genes.
Which of these statements is correct regarding the CRISPR/Cas9 system in eukaryotic cells?
a.Single guide RNA (sgRNA) is used to target specific DNA sequences and activate Cas.
b.Bacteriophages are used to inject viral genes into the cell to activated the Cas protein.
c.CRISPR takes advantage of the cell’s DNA polymerase proof-reading activities for
d.The CRISPR-Cas system can only be used to alter genomes in prokaryotic cells.
e.Cas cuts directly into palindromic restriction sites that it targets in the DNA.
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