You are working as a protein engineer. You decide to work on lacl protein ( This is the repressor protein of the Lac Operon). Through your work you make 2 varieties of lacl. One variety has a lower Kd for the major grove of DNA. The other variety has a lower Kd for allolactose. A. Come up with a name for both varieties of lacl. B. What effect on gene expression might you expect from both varieties of lacl ( You may use the name you came up with in part A to explain both). How might you use these varieties to your advantage to express recombinant proteins
You are working as a protein engineer. You decide to work on lacl protein ( This is the repressor protein of the Lac Operon). Through your work you make 2 varieties of lacl. One variety has a lower Kd for the major grove of DNA. The other variety has a lower Kd for allolactose. A. Come up with a name for both varieties of lacl. B. What effect on gene expression might you expect from both varieties of lacl ( You may use the name you came up with in part A to explain both). How might you use these varieties to your advantage to express recombinant proteins
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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You are working as a protein engineer. You decide to work on lacl protein ( This is the repressor protein of the Lac Operon). Through your work you make 2 varieties of lacl. One variety has a lower Kd for the major grove of DNA. The other variety has a lower Kd for allolactose.
A. Come up with a name for both varieties of lacl.
B. What effect on gene expression might you expect from both varieties of lacl ( You
may use the name you came up with in part A to explain both). How might you use
these varieties to your advantage to express recombinant proteins
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