You find a new mutant strain of E. coli that fails to transcribe the rrnA2 gene, but all other genes are transcribed normally. What type of mutation could lead to this phenotype? A:a mutation affecting the ß-subunit of RNA polymerase B:a deletion of the GC-rich hairpin loop in the rrnA2 3'-UTR C:a mutation affecting rho factor D:a deletion of the -10 region in the rrnA2 promoter E:a mutation affecting sigma facto

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A: Translation is a heterocatalytic action of DNA by means of which RNA is synthesize from specific DNA…

Q: You are studying a human gene, and try to express the protein in E. coli bacterial cells. To do…

A: Cloning is the process of creating a genetically identical copy of a biological entity. Cloning can…

Q: Strain X-23 has a mutation in the lacI gene resulting in a lacI protein that cannot bind DNA.…

A: Gene regulation at the level of transcription in bacteria is achieved by the operon model. Operon…

Q: 1. a)What would happen if the aminoacyl tRNA synthetase responsible for charging alanine tRNAs also…

A: The polymerization of amino acids according to the codons on mRNA to form proteins is known as…

Q: Mutation in the operator that reduces the affinity of the operator for the repressor protein…

A: Lac operon is an inducible operon. When lactose is present in medium, then lac operon starts.

Q: seems that the expert only addressed the background of prorooncogen and the impact of Ras causing…

A: Protooncogenes are the genes that form the proteins that are responsible for cell division and cell…

Q: Geneticists isolated six trains of E. coli (strains 1-6) whose lac operons had one of the following…

A: LAC operon of E. coli is an example of inducible gene expression system. Lactose acts as inducer…

Q: Suppose you had a bacteriophage λ in which the gene encoding the cro protein had been mutated, so…

A: The mutation alters allele frequencies by constantly introducing new alleles, which can be…

Q: E. coli has five genes that code for enzymes that make tryptophan. These genes are regulated by a…

A: When a group of related genes exist under the control of a single promoter, it is referred to as an…

Q: Strain LOLZ has a mutation in the lacI gene that results in a lacI protein that cannot bind…

A: Lac operon is the segment of DNA which is responsible for breaking down of lactose into glucose and…

Q: onsider Figure 3, which shows some features of a eukaryotic gene. A, B, C are exons while 1, 2 are…

A: INTRODUCTION When an RNA transcript is first made during a eukaryotic cell, it's considered a…

Q: Geneticists isolated six trains of E. coli (strains 1-6) whose lac operons had one of the following…

A: Lac operon It is an operon that is needed for the movement and metabolism of lactose in E. coli.

Q: The mRNA used for the SARS-CoV-2 vaccine from Pfizer/BioNTech is generated in a cell-free system by…

A: The hereditary molecule deoxyribonucleic acid (DNA) transmits genetic information from one…

Q: On its chromosome, an E. coli cell has a genotype of lacI− lacZ+ lacY+ lacA+. It has an F′ factor…

A: Lac operon is studied extensively by using mutant strains of E.coli. It is studied under the domain…

Q: Suppose that an investigative team conducted an RNA-Seq experiment on mouse liver cells. They found…

A: DNA sequence segments that straddle the start and stop codons are known as open reading frames…

Q: a. Assume that replication has been initiated at that ORI. Provide the sequence of the primer that…

A: DNA is the genetic material of almost all living organisms. It contains information that is…

Q: You are working in a research lab that is surveying the CFTR gene sequence in humans to identify new…

A: A synonymous mutation is mutation in the DNA sequence that leads to change in the codon for amino…

Q: e following genetic and cellular conditions.  You do not need to consider the role of CAP in your…

A: Upper operon -  Is is super repressor. Once it is present in the sequence, it can not be removed. If…

Q: Would the viral insertion of a proto-oncogene into a region of genomic DNA with non-acetylated…

A: Acetylation reduces the affinity between histones and DNA by removing positive charges. Thus,…

Q: If a wild-type (normal, NOTmutated) E. coli strain is grown in a medium: a. without lactose or…

A: The lac operon or the lactose operon is a mechanism that is involved in the metabolism and transport…

Q: Which of the following will result in an INCREASE in the levels (# of molecules) of the…

A: Enzyme beta-galactosidase (also known as β- gal) is coded by z  gene of  the lac operon. The…

Q: ptoph had a mutation on the repressor, not allowing it to bind with tryptophan. The repressor is…

A: Operons are proteins encoded together as a block that performs specific functions and are involved…

Q: Imagine that a new strain of E. coli is discovered, and it contains the following novel genetic…

A: The lac operon is an operon, which is a collection of genes with a single promoter. The genes in the…

Q: How will this modified cro protein interact with the three OR sites, and how would cro expression be…

A: Bacteriophages are viruses that infect bacteria. Like other viruses bacteriophages are also made up…

Q: You have an E.coli strain in which the lac operon is not expressed when glucose is absent and…

A: Introduction :- E. coli is a bacteria that lives in healthy people and animals' intestines. The…

Q: You have created a null mutation of tRNA synthase for trp-tRNA (the synthase enzyme attaches the trp…

A: A null mutation is a mutation that completely removes a gene or part of a gene.In this case, the…

Q: Does it need active form or inactive form of the repressor ? 3) What is role of RNA polymerase,…

A: An operon is defined as the collection of coordinately regulated genes . Q2Ans:In a negative control…

Q: The following diagram show what is required for an active promoter of a gene of interest, where:A1 =…

A: Based on the given diagram, here are the predictions:Chromatin conformation: EuchromatinMethylation…

Q: 37. Which of the following motifs can be used to bind protein dimers? a.) helix-loop-helix b.) zinc…

A: Motif is nothing but a short conserved sequence pattern, which is associated with protein function…

Q: You have isolated a mutant that you call Mutant Z. In a Mutant Z strain, the RNA polymerase can bind…

A: Here, the correct answer is option c) domain 2 of sigma and d) 3/4 linker of sigma.

Q: In a qPCR experiment, you find that the mRNA for the gene CDK1 goes down (decreased expression) when…

A: qPCR (quantitative polymerase chain reaction), also known as real-time PCR, is a laboratory…

Q: In lac operon, both gene A and gene B undergo a transcription process. Gene B can only undergo…

A: An operon is a gene regulation mechanism in prokaryotes.

Q: The man's genetic defect appears to be due to the mutation of the promoter region of a protein X.…

A: Sanger method is also known as enzymatic chsin termination method and was developed by Frederick…

Q: You are working with a fly hair cell developmental system. This Notch/Delta-regulated system results…

A: In this scenario, a forward genetic screen is conducted in a fly hair cell developmental system to…

Q: Strain P77 has a mutation in the lacO that prevents it from being bound by the lacI protein.…

A: Gene regulation at the level of transcription in bacteria is achieved by the operon model. Operon…

Q: You suspect that the mRNA encoding the guanine biosynthetic genes of Bacillus subtilus contains an…

A: Before we get into figuring out a way to test the above hypothesis, we need to have a good idea on…

Q: ncer where the newly-discovered genes BR and AIN are known to play a role in the development of this…

A: Cancer results from the uncontrolled cell division giving rise to a lump of cells that may be…

Q: In a bacterial cell with the genotype I-: P+: O+: Z+: Y+: A+, which of the following expression…

A: The lac operon in bacterial cells plays a pivotal role in the regulation of lactose metabolism.…

Q: When iron is scarce, some bacteria can stop synthesis of all enzymes that require iron (Fe3+), such…

A: Introduction  Bacteria are single-celled microorganisms that are found almost everywhere on Earth.…

Q: You are studying a disease caused when the gene called BMP5 is not transcribed in osteoblasts. All…

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Mitochondrial Mutations

A mitochondrion is a cellular organelle that serves as the site for cellular respiration and energy production (ATP or Adenosine Triphosphate). It is a double-membrane-bound organelle and is only present in eukaryotic cells.