For each of the pLM160 plasmid elements listed below: define the component AND briefly discuss the functional significance of this component for recombineering in Chlamydomonas reinhardtii. a. APHVIII b. Beta2-tubulin prom c. ccdB
Bacterial Genomics
The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.
Transformation Experiment in Bacteria
In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.
Plasmids and Vectors
The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.
![5' cloning primer
annealing site
3' cloning primer
annealing site
ccdB
ccdB
term
ccdB
CrVenus YFP
prom
KanR2
3xFLAG
prom
PSAD
3'UTR
KanR2
PLM099
5356 bp
HSP70A/RBCS2
prom/5'UTR
KanR2
term
RBCS2
intron
p15A
origin
APHVIII
(paromomycin)
I-Scel recognition site
APHVII (hygromycinⓇ)
PLM161 and 162
BLE (zeocinⓇ)
PLM459
RBCS2 PLM099 and 160
3'UTR
m Turquoise 2-3xHA
PLM459
mScarlet-i-3xFLAG
PLM162
mNeon Green-3xFLAG
PLM160
Beta2-tubulin
prom](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2Fd9761baa-51ab-49d2-8ec7-1bf4df745458%2F448cee08-bfcc-47d3-91f9-e54cc5532a37%2Ftmlmvad_processed.jpeg&w=3840&q=75)
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