Bacteriophage P22 was used in generalized transduction experiments to infect the Salmonella typhimurium donor strains described in the table below. The resulting phage lysates were then used to infect the S. typhimurium recipient strains listed in the table. In each cross, a phenotype was selected for one of the three genetic markers studied (str, aceA, thrA), and then replicates were performed to select the corresponding recombinants for the other two markers. The results are given in the following table: Recipient strain Selected phenotype Selected recombinants Donor strain str thrA aceAt thrA str aceA+ strs thrA+ aceA thrA+ str aceA Str Ace+ Str ThrA ThrA+ ThrA ThrA+ Ace Ace+ str: gene involved in streptomycin resistance, aceA: gene involved in the use of acetate as a carbon source, thrA: gene involved in the biosynthesis of threonine. Number 60 40 95 5 10 90 a. What are the selective media used in these three transduction experiments, on the one hand to obtain the selected phenotypes, on the other hand to obtain the selected recombinants?

Q: Single stranded DNA fragment of M13 phage DNA shown was mixed with the following  primers P1: AGTAG…

A: Polymerase chain reaction also abbreviated as PCR.A specific DNA fragment is quickly multiplied…

Q: T7 promotor in yeast, your protein is a large complex protein. Which would be the ideal vector to…

A: * Given that T7 promotor in yeast has a  p large complex protein.  * An expression vector also…

Q: % Cells Cause Tumors Retrovirus Transformation Assay 120 100 80 60 40 20 0 10 -Virus A -Virus B 20…

A: Transduction is the process by which DNA is transferred from one bacterium to another or to a host…

Q: If you are using a restriction enzyme that make 8 cuts in the Lambda phage DNA, how many bands…

A: Introduction :- Restriction enzymes are bacterial enzymes that cuts the double stranded DNA molecule…

Q: Order the following experimental steps to identify auxotrophic mutants in Saccharomyces Cerevisiae…

A: The objective of the question is to order the steps to identify auxotrophic mutants in Saccharomyces…

Q: All of the following apply to Luria and Delbruck’s 1943 study of mutation rates in E. Coli and T1…

A: The Luria-Delbruck Experiment ---This experiment performed in 1943  demonstrate that in bacteria…

Q: Using blue-white selection, explain how to discriminate against bacteria with self-ligated vectors.…

A: Blue white selection is an efficient technique for the identification of Recombinant bacteria in a…

Q: A conjugation-deficient strain of A. radiobacter is used to combat crown gall disease. Explain how…

A: Disease:   When an organism’s body system does not work properly or any organ affects by viruses,…

Q: All of the following apply to Luria and Delbrűck’s mutation theory as tested using E. Coli and T1…

A: The answer is option c.  All of the following apply to Luria and Delbrűck’s mutation theory as…

Q: What technique could be used to confirm the presence or absence of plasmids in the donor, recipient…

A: Microbiology is the branch of biology that deals with study of organisms that are too small to be…

Q: For your Southern Blot, every band showed a reaction with the probe.Describe what happened and how…

A: Southern Blot is a laboratory technique that is used to detect specific DNA molecules from various…

Q: When using a conventional plasmid cloning vector containing a b-galactosidase gene, it is possible…

A: The blue–white screen is a techinque that allows for the rapid and convenient detection of…

Q: Jerome Vinograd found that when circular DNA from apolyoma virus is subjected to cesium chloride…

A: Polyoma virus is the virus that infect the human and establish symbiotic relationship with humans to…

Q: Once cloning is complete, a recombinant OXA-M290 protein can be produced in E. coli. Competent E.…

A: The addition of IPTG to E. coli BL21(DE3) cells containing the pET-28a-OXA-M290 plasmid induces the…

Q: Which conclusions are consistent with these data? Why?  A) Strains A and B carry mutations in the…

A: Answer :- Option (C) is correct. - Strains B, C, and D carry mutations in the same gene.

Bacterial Genomics

The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.

Transformation Experiment in Bacteria

In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.

Plasmids and Vectors

The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.