A student cloned Claudin 1 (877 bp) gene into pTRE3G-BI-ZsGreen1 (total size is 8905 bp) vector using Not1 and EcoRV restriction sites located at 215bp and 779bp respectively. What is the size of the recombinant plasmid? A) 9782 bp B) 9218 bp C) 10776 bp E) 8028 bp F) None of the answers given
Bacterial Genomics
The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.
Transformation Experiment in Bacteria
In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.
Plasmids and Vectors
The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.
A student cloned Claudin 1 (877 bp) gene into pTRE3G-BI-ZsGreen1 (total size is 8905 bp) vector using Not1 and EcoRV restriction sites located at 215bp and 779bp respectively. What is the size of the recombinant plasmid?
A) 9782 bp
B) 9218 bp
C) 10776 bp
E) 8028 bp
F) None of the answers given
Step by step
Solved in 3 steps
