There may be more than one correct answer, select all that apply.  To clone genes, a plasmid vector should contain a(n) a.) telomeres b.) orgin of replication c.) antibiotic resistance gene d.) restriction endonuclease cut site The CRISPR/Cas system has revolutionized the efficiency at which scientists can experimentally induce a mutation in a gene to investigate the functional role of the protein it encodes. Which of the following are required in the CRISPR/Cas system? Cas9 nuclease Bacterial genome Access to target gene Guide RNA complementary to gene of interest

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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There may be more than one correct answer, select all that apply. 

To clone genes, a plasmid vector should contain a(n)

a.) telomeres

b.) orgin of replication

c.) antibiotic resistance gene

d.) restriction endonuclease cut site

The CRISPR/Cas system has revolutionized the efficiency at which scientists can experimentally induce a mutation in a gene to investigate the functional role of the protein it encodes. Which of the following are required in the CRISPR/Cas system?

  1. Cas9 nuclease
  2. Bacterial genome
  3. Access to target gene
  4. Guide RNA complementary to gene of interest

 

 

Expert Solution
Step 1

Introduction 

A plasmid vector is a small, circular piece of DNA that is used as a tool for transferring genes from one organism to another. It is commonly used in genetic engineering and biotechnology to introduce a specific gene into a host organism, such as bacteria, yeast, or animal cells. The plasmid vector contains the gene of interest, as well as other components that allow for the gene to be replicated and maintained within the host.

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