Pictured below is a map of the pBR322 plasmid vector. If a DNA fragment is inserted into the Sal I site, what can be expected about the growth coli transformants containing the recombinant plasmid? EcoRI Pstl Ampicillin resistance (AmpⓇ) BamHI Tetracycline resistance (TetⓇ) pBR322 (4,361 bp) Sall
Bacterial Genomics
The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.
Transformation Experiment in Bacteria
In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.
Plasmids and Vectors
The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.
![Pictured below is a map of the pBR322 plasmid vector. If a DNA fragment is inserted into the Sal I
site, what can be expected about the growth coli transformants containing the recombinant plasmid?
EcoRI
Pstl
Ampicillin
resistance
(AmpⓇ)
Origin of
replication
(ori)
d. All of the above are true
pBR322
(4,361 bp)
Pvull
BamHI
Tetracycline
resistance
(TetⓇ)
Sall
a. The transformants should grow on nutrient agar containing ampicillin and tetracycline.
b. The transformants should be able to grow on nutrient agar without antibiotics.
c. The transformants should be able to grown on nutrient agar containing ampicillin](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F2950fafb-198b-44f1-a559-ce3dae66bd5f%2F8b3ea0a7-4824-4c11-a645-01b9ed796224%2F95n7i36_processed.png&w=3840&q=75)
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