Protocols in isolating DNA often involve the use of two kinds of ethanol, 100% ethanol and 70% ethanol, in succession. What happens during these steps and why are they essential? 2. Spectrophotometric detection of nucleic acids require readings at wavelengths of 260nm, and 280nm. What is the significance of these wavelengths?
Protocols in isolating DNA often involve the use of two kinds of ethanol, 100% ethanol and 70% ethanol, in succession. What happens during these steps and why are they essential? 2. Spectrophotometric detection of nucleic acids require readings at wavelengths of 260nm, and 280nm. What is the significance of these wavelengths?
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Watch the demonstration video on nucleic acid quantification on the NanoDrop spectrophotometer: https://cutt.ly/bio150dnaquantification
Note: the video is entitled RNA but the method is identical for DNA quantification.
1. Protocols in isolating DNA often involve the use of two kinds of ethanol, 100% ethanol and
70% ethanol, in succession. What happens during these steps and why are they essential?
2. Spectrophotometric detection of
and 280nm. What is the significance of these wavelengths?
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