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What was the direction of centrifugation (which fractions correspond to the bottom of the centrifuge tubes)?

What was the direction of centrifugation (which fractions correspond to the bottom of the centrifuge tubes)?

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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21. What was the direction of centrifugation (which fractions correspond to the bottom of the centrifuge tubes)? Answer:
Transcribed Image Text:21. What was the direction of centrifugation (which fractions correspond to the bottom of the centrifuge tubes)? Answer:
Equilibrium Density Gradient Centrifugation of DNA Samples Review the following terms using the internet before working on the problem: radioactive labeling, DNA, cesium chloride, equilibrium centrifugation, DNA denaturation using heat EXPERIMENT Radioactively labeled bacterial DNA was analyzed by cesium chloride (CSCI) equilibrium density gradient centrifugation. The radioactivity of fractions collected from the gradients, and the densities of the two peak fractions, were determined. Graph A: Native DNA sample; Graph B: DNA sample heated to 100°C and rapidly cooled on ice before centrifugation. FIGURE 1724 1708 10 20 30 40 s0 60 70 SO 10 20 30 40 50 60 70 So FRACTIONS ccessibility: Investigate RADIOACTIVITY
Transcribed Image Text:Equilibrium Density Gradient Centrifugation of DNA Samples Review the following terms using the internet before working on the problem: radioactive labeling, DNA, cesium chloride, equilibrium centrifugation, DNA denaturation using heat EXPERIMENT Radioactively labeled bacterial DNA was analyzed by cesium chloride (CSCI) equilibrium density gradient centrifugation. The radioactivity of fractions collected from the gradients, and the densities of the two peak fractions, were determined. Graph A: Native DNA sample; Graph B: DNA sample heated to 100°C and rapidly cooled on ice before centrifugation. FIGURE 1724 1708 10 20 30 40 s0 60 70 SO 10 20 30 40 50 60 70 So FRACTIONS ccessibility: Investigate RADIOACTIVITY
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