3000 bp 2000 bp 1000 bp 800 bp 700 bp 500 bp L Uncut EcoRI 1 BamHI Ncol EcoR1/BamH1 BamHI/Nco1 Nco/EcoRI B/N/E

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Chapter1: The Human Body: An Orientation
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Use the gel to answer the following questions. You will be constructing a map of the plasmid, pDiddy.

 

IF the EcoRI/BamHI double digest produces 3 fragments with only two sizes, what are their sizes?

 

### Gel Electrophoresis Image Analysis

#### Overview

This image depicts an agarose gel electrophoresis result used to analyze DNA fragments. This method is essential for measuring the size of DNA fragments resulting from various restriction enzyme digestions.

#### Description of Lanes and Samples

- **Lane L**: Represents the DNA ladder, a mixture of DNA fragments of known lengths, which serves as a standard for estimating the sizes of other fragments on the gel.
- **Lane Uncut**: Contains the undigested DNA sample, which appears as a single band.
- **Lane EcoRI**: The DNA sample digested with the restriction enzyme EcoRI.
- **Lane BamHI**: The DNA sample digested with the restriction enzyme BamHI.
- **Lane NcoI**: The DNA sample digested with the restriction enzyme NcoI.
- **Lane BamHI/NcoI**: The DNA sample digested with both BamHI and NcoI.
- **Lane EcoRI/BamHI**: The DNA sample digested with both EcoRI and BamHI.
- **Lane EcoRI/BamHI/NcoI**: The DNA sample digested with EcoRI, BamHI, and NcoI.
- **Lane NcoI/EcoRI**: The DNA sample digested with both NcoI and EcoRI.
- **Lane B/N/E**: The DNA sample digested with BamHI, NcoI, and EcoRI.

#### Measurements

- **Bands Sizes**: The leftmost column, labeled "L," provides the size markers in base pairs (bp): 3000 bp, 2000 bp, 1000 bp, 800 bp, 700 bp, and 500 bp. 
  - Each DNA band within the lanes can be compared against these markers to estimate its size.

#### Analysis

1. **DNA Ladder (Lane L)**:
   - Contains multiple bands at known sizes: 3000 bp, 2000 bp, 1000 bp, 800 bp, 700 bp, and 500 bp. 

2. **Uncut DNA (Lane Uncut)**:
   - Shows a single high molecular weight band indicating the intact DNA without any cuts.

3. **Single Enzyme Digestions**:
   - **EcoRI (Lane EcoRI) and BamHI (Lane BamHI)**: These lanes display patterns of bands that differ according to where each enzyme cuts the
Transcribed Image Text:### Gel Electrophoresis Image Analysis #### Overview This image depicts an agarose gel electrophoresis result used to analyze DNA fragments. This method is essential for measuring the size of DNA fragments resulting from various restriction enzyme digestions. #### Description of Lanes and Samples - **Lane L**: Represents the DNA ladder, a mixture of DNA fragments of known lengths, which serves as a standard for estimating the sizes of other fragments on the gel. - **Lane Uncut**: Contains the undigested DNA sample, which appears as a single band. - **Lane EcoRI**: The DNA sample digested with the restriction enzyme EcoRI. - **Lane BamHI**: The DNA sample digested with the restriction enzyme BamHI. - **Lane NcoI**: The DNA sample digested with the restriction enzyme NcoI. - **Lane BamHI/NcoI**: The DNA sample digested with both BamHI and NcoI. - **Lane EcoRI/BamHI**: The DNA sample digested with both EcoRI and BamHI. - **Lane EcoRI/BamHI/NcoI**: The DNA sample digested with EcoRI, BamHI, and NcoI. - **Lane NcoI/EcoRI**: The DNA sample digested with both NcoI and EcoRI. - **Lane B/N/E**: The DNA sample digested with BamHI, NcoI, and EcoRI. #### Measurements - **Bands Sizes**: The leftmost column, labeled "L," provides the size markers in base pairs (bp): 3000 bp, 2000 bp, 1000 bp, 800 bp, 700 bp, and 500 bp. - Each DNA band within the lanes can be compared against these markers to estimate its size. #### Analysis 1. **DNA Ladder (Lane L)**: - Contains multiple bands at known sizes: 3000 bp, 2000 bp, 1000 bp, 800 bp, 700 bp, and 500 bp. 2. **Uncut DNA (Lane Uncut)**: - Shows a single high molecular weight band indicating the intact DNA without any cuts. 3. **Single Enzyme Digestions**: - **EcoRI (Lane EcoRI) and BamHI (Lane BamHI)**: These lanes display patterns of bands that differ according to where each enzyme cuts the
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